Keck Subbasement:References: Difference between revisions
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*[http://www3.interscience.wiley.com/browse/?type=CURRENT_PROTOCOL Current Protocols] | *[http://www3.interscience.wiley.com/browse/?type=CURRENT_PROTOCOL Current Protocols] | ||
*[http://openwetware.org/wiki/Designing_primers Designing primers] | *[http://openwetware.org/wiki/Designing_primers Designing primers] | ||
* [[Paper on Fusion PCR]] | |||
==Websites== | ==Websites== | ||
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* [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/ IDT primer design] | * [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/ IDT primer design] | ||
* [http://www.microscopyu.com/articles/livecellimaging/fpintro.html Basics on Fluorescent Proteins] | * [http://www.microscopyu.com/articles/livecellimaging/fpintro.html Basics on Fluorescent Proteins] | ||
==Safety== | ==Safety== | ||
Revision as of 14:57, 23 July 2008
Protocols
- Accuprime PCR Protocol
- T4 DNA Ligase
- Quick Ligase for the molar ratio calculation; the ligase we have in lab is the T4 DNA ligase (protocol above)
- XL1-Blue Competent Cells
- Current Protocols
- Designing primers
- Paper on Fusion PCR
Websites
- PubMed for looking up papers
- NetPrimer primer design tool
- Caltech BioControl Reading Group papers on biological control systems
- APh 162 web site - lots of tutorials and protocols
- IDT primer design
- Basics on Fluorescent Proteins
Safety
Pictures
Manuals
- Sony DSC-F707 Cybershot camera (4M PDF)
- Olympus IX71 Inverted Microscope
- Krato (small room) - Motorized stage, X-Cite 120PC illumination source, Hamamatsu camera. Schedule via Google Calendar (send e-mail to RMM to get added)
- Nemesis (dark room 1) - Motorized stage, Lumen 200 illuminiation source, Hamamatsu camera, envrionmental chamber. Schedule via Google Calendar (send e-mail to RMM to get added)
- Kinesin? (on cart) - this microscope showed up in the lab one day. Not sure where it came from. It has a manual stage, X-Cite 120 illumination system and a QImaging camera.
- Prior ProScan II Motorized Stage
- Lumen 200 Fluorescence Illumination System
- X-Cite 120 PC illumination source
- MicroManager 1.x
- Thermo Genesis 10uv spectrophotometer - spec haven't found a user manual yet
- Techne TC-312 thermal cycler
