Keck Subbasement:References: Difference between revisions
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==Protocols== | ==Protocols== | ||
* [http://www.rpgroup.caltech.edu/courses/aph162/2007/Protocols/InvitrogenAccuprimePFXSupermix.pdf Accuprime PCR Protocol] | * [http://www.rpgroup.caltech.edu/courses/aph162/2007/Protocols/InvitrogenAccuprimePFXSupermix.pdf Accuprime PCR Protocol] | ||
*[http://www.neb.com/nebecomm/TechBulletinFiles/techbulletinM0202.pdf T4 DNA Ligase] | |||
*[http://www.neb.com/nebecomm/TechBulletinFiles/techbulletinM2200.pdf Quick Ligase] for the molar ratio calculation; the ligase we have in lab is the T4 DNA ligase (protocol above) | |||
*[http://www.stratagene.com/manuals/200249.pdf XL1-Blue Competent Cells] | |||
*[http://www3.interscience.wiley.com/browse/?type=CURRENT_PROTOCOL Current Protocols] | |||
*[http://openwetware.org/wiki/Designing_primers Designing primers] | |||
*[[Media:Fusion_PCR_intro.pdf | Fusion PCR Intro]] | |||
==Websites== | ==Websites== | ||
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* [http://www.premierbiosoft.com/netprimer/netprlaunch/netprlaunch.html# NetPrimer] primer design tool | * [http://www.premierbiosoft.com/netprimer/netprlaunch/netprlaunch.html# NetPrimer] primer design tool | ||
* [http://openwetware.org/wiki/BioControl Caltech BioControl Reading Group] papers on biological control systems | * [http://openwetware.org/wiki/BioControl Caltech BioControl Reading Group] papers on biological control systems | ||
* [http://www.rpgroup.caltech.edu/courses/aph162/2007 APh 162 web site] - lots of tutorials and protocols | |||
* [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/ primer design] | |||
* [http://www.microscopyu.com/articles/livecellimaging/fpintro.html Basics on Fluorescent Proteins] | |||
* [http://openwetware.org/wiki/E._coli_genotypes Genotypes of E.coli strains] | |||
* [http://www.neb.com/nebecomm/DoubleDigestCalculator.asp Double Digest check] | |||
* [https://www.omegafilters.com/ Omega optical] | |||
* [https://www.idtdna.com/CALTECH/Login.aspx IDT login] | |||
==Safety== | |||
*[http://www.msdssearch.com/DBLinkSN.htm MSDS chemical database] | |||
==Pictures== | ==Pictures== | ||
*1 kb NEB ladder | |||
[[Image:1_kb_ladder.gif]] | [[Image:1_kb_ladder.gif]] | ||
*100 bp NEB ladder | |||
[[Image:N3231_fig1_v1_000034.gif]] | |||
== Manuals == | == Manuals == | ||
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** [http://www.hyphenated-systems.com/content/support/X-Cite120PCuserguide.pdf X-Cite 120 PC illumination source] | ** [http://www.hyphenated-systems.com/content/support/X-Cite120PCuserguide.pdf X-Cite 120 PC illumination source] | ||
** MicroManager 1.x | ** MicroManager 1.x | ||
* Thermo Genesis 10uv spectrophotometer - haven't found a user manual yet | * Thermo Genesis 10uv spectrophotometer - [http://www.thermo.com/com/cda/product/detail/1,1055,12624,00.html spec] haven't found a user manual yet | ||
* [http://www.techneusa.com/support/manuals/tc-312.pdf Techne TC-312 thermal cycler] | * [http://www.techneusa.com/support/manuals/tc-312.pdf Techne TC-312 thermal cycler] | ||
Latest revision as of 12:57, 18 February 2009
Protocols
- Accuprime PCR Protocol
- T4 DNA Ligase
- Quick Ligase for the molar ratio calculation; the ligase we have in lab is the T4 DNA ligase (protocol above)
- XL1-Blue Competent Cells
- Current Protocols
- Designing primers
- Fusion PCR Intro
Websites
- PubMed for looking up papers
- NetPrimer primer design tool
- Caltech BioControl Reading Group papers on biological control systems
- APh 162 web site - lots of tutorials and protocols
- primer design
- Basics on Fluorescent Proteins
- Genotypes of E.coli strains
- Double Digest check
- Omega optical
- IDT login
Safety
Pictures
- 1 kb NEB ladder
- 100 bp NEB ladder
Manuals
- Sony DSC-F707 Cybershot camera (4M PDF)
- Olympus IX71 Inverted Microscope
- Krato (small room) - Motorized stage, X-Cite 120PC illumination source, Hamamatsu camera. Schedule via Google Calendar (send e-mail to RMM to get added)
- Nemesis (dark room 1) - Motorized stage, Lumen 200 illuminiation source, Hamamatsu camera, envrionmental chamber. Schedule via Google Calendar (send e-mail to RMM to get added)
- Kinesin? (on cart) - this microscope showed up in the lab one day. Not sure where it came from. It has a manual stage, X-Cite 120 illumination system and a QImaging camera.
- Prior ProScan II Motorized Stage
- Lumen 200 Fluorescence Illumination System
- X-Cite 120 PC illumination source
- MicroManager 1.x
- Thermo Genesis 10uv spectrophotometer - spec haven't found a user manual yet
- Techne TC-312 thermal cycler
