Kelley:CommonArea: Difference between revisions

Revision as of 10:24, 8 February 2012

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Do not leave your items in the sink. Any items left around sink may be thrown away.
Wipe down counter to keep area clean and dry

Turn on with probe still in buffer
Remove probe from buffer and rinse with ddH2O into rinse waste collection container
Measure pH
Rinse again after use
Replace probe in buffer before turning off
Empty rinse waste collection container
Clean up any mess you make

@@ Line 28: / Line 28: @@
 * Empty rinse waste collection container
 * Clean up any mess you make
-==Gel Area==
-After running gel electrophoresis
-#Turn off the UV lamp in the imager.  Remove the pipette tip from the safety trigger.
-#Remove the gel from the imager. To recycle the agarose gel, place it in one of the two designated 500mL containers (with purple caps) located in the gel area.
-#To dispose the agarose gel, dump it into the five gallon black gel solid waste container located on the ground near the -80 freezer.
-#Dispose the plastic wrap into regular biological waste and razor blade into the bench-top red sharps container.
-#Used electrophoresis buffer can be directly dumped into the sink.  To reuse the buffer, leave it in the gel box and cover the gel box to prevent evaporation.
-#Rinse the plastic gel tray and dry it in the sink area.
-#Measuring cylinders filled with buffer should be cover by Parafilm to prevent evaporation. Remove any empty measuring cylinders from the area.
-#Clean up any spills immediately.
-==Plate Reader==
-* Remove the microplate from the platereader after use. Don’t leave used microplates in the surrounding area.
-* Please DO NOT grow (with shaking) your cell cultures in the platereader. The platereader is not designed for cell culturing.