How do internal and external environments control the transmission of cellular information? Our lab is investigating the mechanisms by which oxidative extracellular environments, such as those found in inflammation, and intracellular oxidation initiated by receptor ligation influence the ability of cells to signal properly. We are characterizing proteomic thiol oxidation through a strong synergy between computational and experimental methods. We use computational modeling to study how signaling networks may be regulated by changes in protein activities due to thiol modifications arising from intra- or extracellular produced reactive oxygen species. Experimentally, we are developing novel high-throughput biochemical assays to detect and quantify the S-glutathionylation and oxidation of proteins.