How do internal and external environments control the transmission of cellular information? Our lab is investigating the mechanisms by which extracellular oxidation (by inflammation), and intracellular oxidation (such as initiated by receptor ligation) influence the ability of cells to signal properly. We are characterizing proteomic dynamics of thiol oxidation through a strong synergy between computational and experimental methods. We use computational modeling to study how signaling networks may be regulated by changes in protein activities due to thiol modifications arising from reactive oxygen species. Experimentally, we are developing novel high-throughput biochemical assays to detect and quantify the S-glutathionylation and oxidation of proteins.