How do internal and external environments control the transmission of cellular information? Our lab is investigating the mechanisms by which extracellular oxidation (by inflammation), and intracellular oxidation (such as initiated by receptor ligation) influence the ability of cells to signal. We rely upon a strong synergy between computational and experimental methods to characterize proteomic dynamics of thiol oxidation. Because of the numerous biochemical reactions involved, we use computational modeling to investigate how signaling networks are regulated by redox-sensitive proteins, whose activities change in the presence of reactive oxygen species. Experimentally, we are developing novel high-throughput biochemical assays to detect and quantify the S-glutathionylation and oxidation of proteins.