Kemp:Research: Difference between revisions
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Aberrations in redox potential are associated with cancerous phenotypes, resulting in a resistance towards chemotherapeutic drugs. Reactive oxygen species (ROS) such as hydrogen peroxide and superoxide are generated by ligation events across a diverse range of receptor families; redox couples provide a means of translating the presence of ROS into useful signals in the cell. Thioredoxin and glutathione-mediated post-translational modifications of proteins (thiolation and glutathionylation, respectively) have been shown to functionally alter the activity of certain proteins. However, few proteins have been investigated in depth to understand this relationship. More broadly, an in-depth quantitative analysis of how redox-related effects systemically influence the regulation of a receptor signaling pathway has never been undertaken. Challenges in quantifying post-translational events and discerning the effects of one redox couple from another have compounded the difficulties in understanding the role of redox-potential in cellular signaling, mandating a modeling-based approach for gaining insight into these biological processes. | Aberrations in redox potential are associated with cancerous phenotypes, resulting in a resistance towards chemotherapeutic drugs. Reactive oxygen species (ROS) such as hydrogen peroxide and superoxide are generated by ligation events across a diverse range of receptor families; redox couples provide a means of translating the presence of ROS into useful signals in the cell. Thioredoxin and glutathione-mediated post-translational modifications of proteins (thiolation and glutathionylation, respectively) have been shown to functionally alter the activity of certain proteins. However, few proteins have been investigated in depth to understand this relationship. More broadly, an in-depth quantitative analysis of how redox-related effects systemically influence the regulation of a receptor signaling pathway has never been undertaken. Challenges in quantifying post-translational events and discerning the effects of one redox couple from another have compounded the difficulties in understanding the role of redox-potential in cellular signaling, mandating a modeling-based approach for gaining insight into these biological processes. | ||
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The Kemp lab uses computational modeling and wet-lab experimentation to investigate how thiolation/ | The Kemp lab uses computational modeling and wet-lab experimentation to investigate how thiolation/glutathionylation of proteins influences the information flow from receptors to the nucleus. We study these effects in the context of T cell activation through TCR ligation. Research projects include: <br> | ||
* modeling of NF-kB regulation through | * modeling of NF-kB regulation through redox couples in pediatric acute lymphoblastic leukemia (with Harry Findley, Children's Healthcare of Atlanta and Emory School of Medicine) <br> | ||
* development of new techniques to monitor | * development of new techniques to monitor glutathionylation of proteins <br> | ||
* modeling systemic influences of | * modeling systemic influences of endogenous hydrogen peroxide on cellular phosphorylation levels | ||
* development of microfluidic devices for capturing fast dynamics of T cell signaling (with Hang Lu, Georgia Tech) | |||
Revision as of 10:16, 9 August 2007
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Aberrations in redox potential are associated with cancerous phenotypes, resulting in a resistance towards chemotherapeutic drugs. Reactive oxygen species (ROS) such as hydrogen peroxide and superoxide are generated by ligation events across a diverse range of receptor families; redox couples provide a means of translating the presence of ROS into useful signals in the cell. Thioredoxin and glutathione-mediated post-translational modifications of proteins (thiolation and glutathionylation, respectively) have been shown to functionally alter the activity of certain proteins. However, few proteins have been investigated in depth to understand this relationship. More broadly, an in-depth quantitative analysis of how redox-related effects systemically influence the regulation of a receptor signaling pathway has never been undertaken. Challenges in quantifying post-translational events and discerning the effects of one redox couple from another have compounded the difficulties in understanding the role of redox-potential in cellular signaling, mandating a modeling-based approach for gaining insight into these biological processes.
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