Ketner Lab

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Current revision (15:55, 20 April 2008) (view source)
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== Current Projects ==
== Current Projects ==
'''Adenovirus Vaccines'''
'''Adenovirus Vaccines'''
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*Malaria
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*[[Malaria]]
*HPV
*HPV
'''Ubiquination'''
'''Ubiquination'''
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-
----
 
== Protocols ==
== Protocols ==
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*Calculate MOI
*Calculate MOI
*Protein preps of infected 293 cells for westerns
*Protein preps of infected 293 cells for westerns
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*Western Blots
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*[http://www.openwetware.org/index.php?title=Ketner:_Tissue_culture_viral_lysate_Western_Blot Western Blot]
*P32 label DNA
*P32 label DNA
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*Quantitate number of virions in CsCl virus preps
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 +
==Materials==
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===Antibodies===
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*[[anti-HA]]
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*[[anti-Fiber]]
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*[[anti-Late serum]]
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*[[anti-NANP hybridoma supernatant]]
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===Plasmids===
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===Viruses===
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===Cells===
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 +
== Links ==
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[http://faculty.jhsph.edu/?F=Gary&L=Ketner Gary Ketner]
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 +
[http://www.jhsph.edu/PublicHealthNews/Magazine/ JHSPH Magazine]

Current revision

Image:JHSPHbanner.jpg


Contents

Current Projects

Adenovirus Vaccines

Ubiquination


Protocols

  • Adenovirus CaCl transfection
  • Generating hexon mutants in E. coli
  • Generating fiber mutants in E. coli
  • Generating late gene expression mutants in 293 cells
  • Purifying adenovius (banded stocks)
  • Titration of viral stocks (calculate pfu)
  • Calculate MOI
  • Protein preps of infected 293 cells for westerns
  • Western Blot
  • P32 label DNA
  • Quantitate number of virions in CsCl virus preps

Materials

Antibodies

Plasmids

Viruses

Cells

Links

Gary Ketner

JHSPH Magazine

Personal tools