Ketner Lab

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (14:55, 20 April 2008) (view source)
m
 
(6 intermediate revisions not shown.)
Line 20: Line 20:
*Calculate MOI
*Calculate MOI
*Protein preps of infected 293 cells for westerns
*Protein preps of infected 293 cells for westerns
-
*Western Blots
+
*[http://www.openwetware.org/index.php?title=Ketner:_Tissue_culture_viral_lysate_Western_Blot Western Blot]
*P32 label DNA
*P32 label DNA
 +
*Quantitate number of virions in CsCl virus preps
 +
==Materials==
 +
===Antibodies===
 +
*[[anti-HA]]
 +
*[[anti-Fiber]]
 +
*[[anti-Late serum]]
 +
*[[anti-NANP hybridoma supernatant]]
 +
 +
===Plasmids===
 +
===Viruses===
 +
===Cells===
== Links ==
== Links ==

Current revision

Image:JHSPHbanner.jpg


Contents

Current Projects

Adenovirus Vaccines

Ubiquination


Protocols

  • Adenovirus CaCl transfection
  • Generating hexon mutants in E. coli
  • Generating fiber mutants in E. coli
  • Generating late gene expression mutants in 293 cells
  • Purifying adenovius (banded stocks)
  • Titration of viral stocks (calculate pfu)
  • Calculate MOI
  • Protein preps of infected 293 cells for westerns
  • Western Blot
  • P32 label DNA
  • Quantitate number of virions in CsCl virus preps

Materials

Antibodies

Plasmids

Viruses

Cells

Links

Gary Ketner

JHSPH Magazine

Personal tools