Ketner Lab
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*Calculate MOI | *Calculate MOI | ||
*Protein preps of infected 293 cells for westerns | *Protein preps of infected 293 cells for westerns | ||
| - | *[ | + | *[http://www.openwetware.org/index.php?title=Ketner:_Tissue_culture_viral_lysate_Western_Blot Western Blot] |
*P32 label DNA | *P32 label DNA | ||
| + | *Quantitate number of virions in CsCl virus preps | ||
==Materials== | ==Materials== | ||
===Antibodies=== | ===Antibodies=== | ||
| - | *anti-HA | + | *[[anti-HA]] |
| - | *anti-Fiber | + | *[[anti-Fiber]] |
| - | *anti-Late serum | + | *[[anti-Late serum]] |
| - | *anti-NANP hybridoma supernatant | + | *[[anti-NANP hybridoma supernatant]] |
| + | |||
| + | ===Plasmids=== | ||
| + | ===Viruses=== | ||
| + | ===Cells=== | ||
== Links == | == Links == | ||
Current revision
Contents |
Current Projects
Adenovirus Vaccines
- Malaria
- HPV
Ubiquination
Protocols
- Adenovirus CaCl transfection
- Generating hexon mutants in E. coli
- Generating fiber mutants in E. coli
- Generating late gene expression mutants in 293 cells
- Purifying adenovius (banded stocks)
- Titration of viral stocks (calculate pfu)
- Calculate MOI
- Protein preps of infected 293 cells for westerns
- Western Blot
- P32 label DNA
- Quantitate number of virions in CsCl virus preps
