Ketner Lab: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
mNo edit summary |
||
| Line 22: | Line 22: | ||
*[http://www.openwetware.org/index.php?title=Ketner:_Tissue_culture_viral_lysate_Western_Blot Western Blot] | *[http://www.openwetware.org/index.php?title=Ketner:_Tissue_culture_viral_lysate_Western_Blot Western Blot] | ||
*P32 label DNA | *P32 label DNA | ||
* | *Quantitate number of virions in CsCl virus preps | ||
==Materials== | ==Materials== | ||
Latest revision as of 12:55, 20 April 2008
Current Projects
Adenovirus Vaccines
- Malaria
- HPV
Ubiquination
Protocols
- Adenovirus CaCl transfection
- Generating hexon mutants in E. coli
- Generating fiber mutants in E. coli
- Generating late gene expression mutants in 293 cells
- Purifying adenovius (banded stocks)
- Titration of viral stocks (calculate pfu)
- Calculate MOI
- Protein preps of infected 293 cells for westerns
- Western Blot
- P32 label DNA
- Quantitate number of virions in CsCl virus preps
