Ketner Lab

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 10: Line 10:
'''Ubiquination'''
'''Ubiquination'''
-
 
-
----
 
== Protocols ==
== Protocols ==
Line 24: Line 22:
*Western Blots
*Western Blots
*P32 label DNA
*P32 label DNA
 +
 +
 +
== Links ==
 +
[http://faculty.jhsph.edu/?F=Gary&L=Ketner Gary Ketner]
 +
 +
[http://www.jhsph.edu/PublicHealthNews/Magazine/ JHSPH Magazine]

Revision as of 09:38, 30 August 2006

Image:JHSPHbanner.jpg


Current Projects

Adenovirus Vaccines

  • Malaria
  • HPV

Ubiquination


Protocols

  • Adenovirus CaCl transfection
  • Generating hexon mutants in E. coli
  • Generating fiber mutants in E. coli
  • Generating late gene expression mutants in 293 cells
  • Purifying adenovius (banded stocks)
  • Titration of viral stocks (calculate pfu)
  • Calculate MOI
  • Protein preps of infected 293 cells for westerns
  • Western Blots
  • P32 label DNA


Links

Gary Ketner

JHSPH Magazine

Personal tools