Ketner Lab: Difference between revisions
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'''Ubiquination''' | '''Ubiquination''' | ||
== Protocols == | == Protocols == | ||
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*Western Blots | *Western Blots | ||
*P32 label DNA | *P32 label DNA | ||
== Links == | |||
[http://faculty.jhsph.edu/?F=Gary&L=Ketner Gary Ketner] | |||
[http://www.jhsph.edu/PublicHealthNews/Magazine/ JHSPH Magazine] | |||
Revision as of 07:38, 30 August 2006
Current Projects
Adenovirus Vaccines
- Malaria
- HPV
Ubiquination
Protocols
- Adenovirus CaCl transfection
- Generating hexon mutants in E. coli
- Generating fiber mutants in E. coli
- Generating late gene expression mutants in 293 cells
- Purifying adenovius (banded stocks)
- Titration of viral stocks (calculate pfu)
- Calculate MOI
- Protein preps of infected 293 cells for westerns
- Western Blots
- P32 label DNA
