Ketner Lab

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(Protocols)
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*[[Western Blots]]
*[[Western Blots]]
*P32 label DNA
*P32 label DNA
 +
 +
==Materials==
 +
===Antibodies===
 +
*anti-HA
 +
*anti-Fiber
 +
*anti-Late serum
 +
*anti-NANP hybridoma supernatant
== Links ==
== Links ==

Revision as of 12:17, 6 September 2006

Image:JHSPHbanner.jpg


Contents

Current Projects

Adenovirus Vaccines

Ubiquination


Protocols

  • Adenovirus CaCl transfection
  • Generating hexon mutants in E. coli
  • Generating fiber mutants in E. coli
  • Generating late gene expression mutants in 293 cells
  • Purifying adenovius (banded stocks)
  • Titration of viral stocks (calculate pfu)
  • Calculate MOI
  • Protein preps of infected 293 cells for westerns
  • Western Blots
  • P32 label DNA

Materials

Antibodies

  • anti-HA
  • anti-Fiber
  • anti-Late serum
  • anti-NANP hybridoma supernatant

Links

Gary Ketner

JHSPH Magazine

Personal tools