Ketner Lab

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(Materials)
Line 22: Line 22:
*[http://www.openwetware.org/index.php?title=Ketner:_Tissue_culture_viral_lysate_Western_Blot Western Blot]
*[http://www.openwetware.org/index.php?title=Ketner:_Tissue_culture_viral_lysate_Western_Blot Western Blot]
*P32 label DNA
*P32 label DNA
 +
*Quantate number of virions in CsCl virus preps
==Materials==
==Materials==

Revision as of 11:05, 15 November 2006

Image:JHSPHbanner.jpg


Contents

Current Projects

Adenovirus Vaccines

Ubiquination


Protocols

  • Adenovirus CaCl transfection
  • Generating hexon mutants in E. coli
  • Generating fiber mutants in E. coli
  • Generating late gene expression mutants in 293 cells
  • Purifying adenovius (banded stocks)
  • Titration of viral stocks (calculate pfu)
  • Calculate MOI
  • Protein preps of infected 293 cells for westerns
  • Western Blot
  • P32 label DNA
  • Quantate number of virions in CsCl virus preps

Materials

Antibodies

Plasmids

Viruses

Cells

Links

Gary Ketner

JHSPH Magazine

Personal tools