Ketner Lab: Difference between revisions
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*Calculate MOI | *Calculate MOI | ||
*Protein preps of infected 293 cells for westerns | *Protein preps of infected 293 cells for westerns | ||
*[ | *[http://www.openwetware.org/index.php?title=Ketner:_Tissue_culture_viral_lysate_Western_Blot Western Blot] | ||
*P32 label DNA | *P32 label DNA | ||
Revision as of 11:01, 6 September 2006
Current Projects
Adenovirus Vaccines
- Malaria
- HPV
Ubiquination
Protocols
- Adenovirus CaCl transfection
- Generating hexon mutants in E. coli
- Generating fiber mutants in E. coli
- Generating late gene expression mutants in 293 cells
- Purifying adenovius (banded stocks)
- Titration of viral stocks (calculate pfu)
- Calculate MOI
- Protein preps of infected 293 cells for westerns
- Western Blot
- P32 label DNA
