Ketner Lab
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Current Projects
Adenovirus Vaccines
- Malaria
- HPV
Ubiquination
Protocols
- Adenovirus CaCl transfection
- Generating hexon mutants in E. coli
- Generating fiber mutants in E. coli
- Generating late gene expression mutants in 293 cells
- Purifying adenovius (banded stocks)
- Titration of viral stocks (calculate pfu)
- Calculate MOI
- Protein preps of infected 293 cells for westerns
- Western Blots
- P32 label DNA
Materials
Antibodies
- anti-HA
- anti-Fiber
- anti-Late serum
- anti-NANP hybridoma supernatant
