Kevin T.L. Vogelsang: Difference between revisions
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(New page: ==Kevin T.L. Vogelsang== MIT Class of 2009 *Hometown: Paintsville, Kentucky *Major: Biological Engineering, Electrical Engineering and Computer Science) |
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<center> | |||
{| border="1" | |||
! Plate | |||
! Expected | |||
! Observed | |||
|- | |||
|- | |||
| 1) (-) No DNA | |||
| none | |||
| none | |||
|- | |||
| 2) (+) untreated plasmid | |||
| lots | |||
| 624 | |||
|- | |||
| 3) backbone + kilcut (- ligase) | |||
| none | |||
| none | |||
|- | |||
| 4) backbone + kilcut (+ ligase) | |||
| none | |||
| none | |||
|- | |||
| 5) backbone +kilcut +ligase + insert | |||
| lots | |||
| 7 | |||
|- | |||
! Transformation Efficiency: (1.25 e5) colonies per microgram | |||
|} | |||
</center> | |||
<br> | |||
Ubp8 | |||
Forward Primer: uppercase: Ubp8 tail italicized lowercase: landing region | |||
TACTTGAAACCCTGCTTTTTTTATTTGTTATTAATAATTatgtcgaaagctacatataa | |||
Top strand of full ‘reverse’ primer: capital: tail italicized lower case: landing sequence | |||
5’ TTCAGCAAATAGCATTCAACAATAATAATAAAACAAAAAgatgcggccagcaaaactaa3’ | |||
Latest revision as of 23:03, 3 April 2007
| Plate | Expected | Observed |
|---|---|---|
| 1) (-) No DNA | none | none |
| 2) (+) untreated plasmid | lots | 624 |
| 3) backbone + kilcut (- ligase) | none | none |
| 4) backbone + kilcut (+ ligase) | none | none |
| 5) backbone +kilcut +ligase + insert | lots | 7 |
| Transformation Efficiency: (1.25 e5) colonies per microgram |
Ubp8
Forward Primer: uppercase: Ubp8 tail italicized lowercase: landing region
TACTTGAAACCCTGCTTTTTTTATTTGTTATTAATAATTatgtcgaaagctacatataa
Top strand of full ‘reverse’ primer: capital: tail italicized lower case: landing sequence 5’ TTCAGCAAATAGCATTCAACAATAATAATAAAACAAAAAgatgcggccagcaaaactaa3’
