Kevin T.L. Vogelsang
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Plate | plasmid with insert | plasmid no insert | ||
---|---|---|---|---|
Expected | ZraI | BamHI | ||
Observed | SEBuffer B NEBuffer 1, 2, 4 | NEBuffer 2 | ||
Temperature | 37 C | 37 C | ||
Predicted fragments | 1 DNA fragment the length of the M13 backbone plus the insert | 1 Fragment the lenght of the original M13 genome | ||
Expected | Observed | |||
Enzyme(s) used | BamHI | ZraI | ||
Buffer used | NEBuffer 2 | SEBuffer B; NEBuffer 1,2,4 | ||
Temperature | 37 C | 37 C | ||
Predicted fragments | No linear fragments. Plasmid remains circular. | No linear fragments. Plasmid remains circular. |