Kevin T.L. Vogelsang

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Plate Expected Observed
1) (-) No DNA none none
2) (+) untreated plasmid lots 624
3) backbone + kilcut (- ligase) none none
4) backbone + kilcut (+ ligase) none none
5) backbone +kilcut +ligase + insert lots 7
Transformation Efficiency: (1.25 e5) colonies per microgram


Ubp8

Forward Primer: uppercase: Ubp8 tail italicized lowercase: landing region

TACTTGAAACCCTGCTTTTTTTATTTGTTATTAATAATTatgtcgaaagctacatataa

Top strand of full ‘reverse’ primer: capital: tail italicized lower case: landing sequence 5’ TTCAGCAAATAGCATTCAACAATAATAATAAAACAAAAAgatgcggccagcaaaactaa3’

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