Kim:Internal: Difference between revisions

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[[Kim:News | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''NEWS''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:News | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''NEWS''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:Links | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''LINKS''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:Links | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''LINKS''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:Contact | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''CONTACT''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:Outreach | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''OUTREACH''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[[Kim:Contact | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''CONTACT''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:Outreach | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''OUTREACH''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
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<table border="1">
<table border="1">
<th>Date</th><th>Presenters</th>
<th>Date</th><th>Presenters</th>
<tr><td>09/14/2012</td><td>No meeting</td></tr>
<tr><td>01/15/2013</td><td>Hyok, Young Soo</td></tr>
<tr><td>09/21/2012</td><td>Julie, Derek, Cameron & Jungho</td></tr>
<tr><td>01/22/2013</td><td>Jinah</td></tr>
<tr><td>09/28/2012</td><td>Kshitiz, Daniel & Anna</td></tr>
<tr><td>01/29/2013</td><td>Hee Seok</td></tr>
<tr><td>10/05/2012</td><td>Dr. Kwan-Hwi Lee & Nicole</td></tr>
<tr><td>02/05/2013</td><td>Kihwan</td></tr>
<tr><td>10/12/2012</td><td>Hyok, Katya & Nicholas</td></tr>
<tr><td>02/12/2013</td><td>Daniel, Sam</td></tr>
<tr><td>10/19/2012</td><td>Jesse & Jinah</td></tr>
<tr><td>02/19/2013</td><td>Kshitiz</td></tr>
<tr><td>10/24/2012</td><td>Ki-Hwan & Peter</td></tr>
<tr><td>02/26/2013</td><td>Jon</td></tr>
<tr><td>11/1/2012</td><td>Alex, Hee-Seok</td></tr>
<tr><td>03/05/2013</td><td>Nick, Julie</td></tr>
<tr><td>11/9/2012</td><td>Sam, David</td></tr>
<tr><td>03/12/2013</td><td>Nicole</td></tr>
<tr><td>11/16/2012</td><td>Kshitiz, Hyok</td></tr>
<tr><td>03/19/2013</td><td>Jesse</td></tr>
<tr><td>11/20/2012</td><td>Katya, Daniel</td></tr>
<tr><td>03/26/2013</td><td>Alex</td></tr>
<tr><td>11/30/2012</td><td>Andrew, Jonathan, Anna, Peter</td></tr>
<tr><td>12/7/2012</td><td>Jesse, Jinah</td></tr>
</table>
</table>
<br>
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==Lab Jobs==
==Lab Jobs==
[https://docs.google.com/a/uw.edu/document/d/1Xw_gHpacpjQZTpsDJgi-g714FzEZCwjl7xjT4ArenVA/edit SLU undergraduate checklist]
<br><br>
<br><br>
'''Autoclaving'''
[https://docs.google.com/a/uw.edu/document/d/1Rsy0nKSwBWJjQEFaJTBljsj90OISXEWCSlnT1_F3nIA/edit Foege undergraduate checklist]
<br>
 
'''SLU:''' microcentrifuge tubes, glass aspirating pipettes, glassware (as needed)Pack items into autoclave bags, seal with tape and label “Kim Lab 419” Take items down to autoclave facility in basement.
==Resources==
<br>
Ottino JM.  "Is a picture worth 1,000 words?" <b> Nature </b> Vol. 421, No. 6922, 474-476, 2003. [http://www.nature.com/nature/journal/v421/n6922/pdf/421474a.pdf Article]<br>
'''Foege:''' microcentrifuge tubes, glass aspirating pipettes, Water bottles Pack items into autoclave bags, seal with tape. Run autoclave items (apply autoclave tapes) Run autoclave biohazard waste (apply autoclave tapes.)
Senturia, SD. "How to Avoid the Reviewer's Axe: One Editor's View." <b>IEEE Transactions on Ultrasonics Ferroelectrics and Frequency Control</b> Vol. 51, No. 1, 127, 2004 [http://web.mit.edu/andrew3/Public/Papers/2004/Senturia/2004_IEEE%20Transactions%20on%20Ultrasonics%20Perroelectrics%20and%20Frequency%20Control_How%20to%20Avoid%20the%20Reviewer%E2%80%99s_Senturia.pdf Article]<br>
<br><br>
Whitesides, GM. "Whitesides' group: writing a paper." <b>Advanced Materials</b> Vol. 16, No. 15, 1375-1377, 2004. [http://chemistry.bd.psu.edu/justik/CHEM%20400/CHEM%20400%20Writing%20a%20Paper.pdf Article] <br>
''' TC Maintenence'''
<br>
'''SLU:''' Fill ethanol supply (dilute 190 proof w/DI water). Refill pipettes in drawers and note remaining supply. Notify Melissa or place order for any materials running low such as pipettes, sterile culture dishes or flasks. Take out biohazard waste to autoclave room (mark “DH Kim 419” and apply autoclave tape). Fold pipette keeper boxes as needed.
<br>
'''Foege:''' Refill pipettes and tubes in drawers and note remaining supply. Fill autoclaved water (approx 2L) of tray in incubator. Fold pipette keeper boxes as needed. Fill ethanol supply (dilute 190 proof w/DI water). Notify Melissa or place order for any materials running low such as pipettes, tubes, dishes, cover glasses, sterile culture dishes or flasks.  
<br><br>
''' Stocking and Aliquoting'''
<br>
'''SLU:''' Remove a stock bottle from the freezer and place in 4C overnight to thaw. Check supply remaining and place order for more if needed. Trypsin and Pen Strep should be aliquoted into centrifuge tubes at 10mL, and FBS in 25mL. Label clearly and refreeze.
<br>
'''Foege:''' Trypsin, P/S, FBS<br>
Remove a stock bottle from the freezer and place in 4C overnight to thaw. Check supply remaining and place order for more if needed. Trypsin and Pen Strep should be aliquoted into centrifuge tubes at 10mL, and FBS in 25mL. Label clearly and refreeze.  
<br><br>
''' Water Bath'''
<br> Drain water, spray down and wipe with ethanol (be sure to lift bottom plating to clean heating coils). Refill (approx 10L) and add appropriate amount of blue water bath solution.
<br><br>
'''Eye Wash'''<br>
Verify that the stations (by the main door and in TC room at SLU, front sink at Foege) are functioning properly.


==Important Links==
==Important Links==


 
*[https://docs.google.com/document/d/1U_71qkVoRCsC45oxgasnQzic21aF3TdBPSjgeOQnBy8/edit Lab contact list] <br>
* [https://www.google.com/calendar/render?tab=oc Microscope Schedule] <br>
*[https://docs.google.com/document/d/1m5A0TNLGMGf_5X9Ld2oXNM2lmE-KsPVXOikyDgQ_9ig/edit#heading=h.gjdgxs Weekly report] <br>
 
*[https://www.google.com/calendar/render?tab=oc Microscope Schedule] <br>
* [https://docs.google.com/a/uw.edu/spreadsheet/ccc?key=0At43xqZHCbEQdHpweGlrbVo0aktmVE5HNnlQZS1CQmc#gid=0 Order Sheet] <br>
*[https://docs.google.com/a/uw.edu/spreadsheet/ccc?key=0At43xqZHCbEQdHpweGlrbVo0aktmVE5HNnlQZS1CQmc#gid=0 Order Sheet] <br>
*[https://docs.google.com/spreadsheet/ccc?key=0AlqhPQ3eN3okdG1SOFFjMDVPODJfTkY3elJJT19ocXc#gid=0 Antibody Inventory] <br>
*[https://docs.google.com/spreadsheet/ccc?key=0At43xqZHCbEQdDkybGhWbzRmck5XZ1YzY05vTFNQZGc#gid=2 Liquid Nitrogen Inventory]

Revision as of 00:02, 19 March 2013

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Meeting Schedule

Group meetings in Foege N403 (3:00pm-5:00pm)

DatePresenters
01/15/2013Hyok, Young Soo
01/22/2013Jinah
01/29/2013Hee Seok
02/05/2013Kihwan
02/12/2013Daniel, Sam
02/19/2013Kshitiz
02/26/2013Jon
03/05/2013Nick, Julie
03/12/2013Nicole
03/19/2013Jesse
03/26/2013Alex


Protocols


Lab Jobs

SLU undergraduate checklist

Foege undergraduate checklist

Resources

Ottino JM. "Is a picture worth 1,000 words?" Nature Vol. 421, No. 6922, 474-476, 2003. Article
Senturia, SD. "How to Avoid the Reviewer's Axe: One Editor's View." IEEE Transactions on Ultrasonics Ferroelectrics and Frequency Control Vol. 51, No. 1, 127, 2004 Article
Whitesides, GM. "Whitesides' group: writing a paper." Advanced Materials Vol. 16, No. 15, 1375-1377, 2004. Article

Important Links

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