Kim:Internal: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(21 intermediate revisions by 4 users not shown)
Line 10: Line 10:
[[Kim:News | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''NEWS''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:News | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''NEWS''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:Links | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''LINKS''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:Links | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''LINKS''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:Contact | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''CONTACT''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:Outreach | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''OUTREACH''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[[Kim:Contact | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''CONTACT''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:Outreach | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''OUTREACH''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
</center>
</center>
</div><br>
</div><br>
Line 19: Line 19:


==Meeting Schedule==
==Meeting Schedule==
Group meetings in Foege N403 (3:00pm-5:00pm) <br>
Fall Quarter 2013 Group meetings Wednesdays in Foege N403 (3:30pm) <br>


<table border="1">
<table border="1">
<th>Date</th><th>Presenters</th>
<th>Date</th><th>Presenters</th>
<tr><td>09/14/2012</td><td>No meeting</td></tr>
<tr><td>10/02/2013</td><td>Cameron</td></tr>
<tr><td>09/21/2012</td><td>Julie, Derek, Cameron & Jungho</td></tr>
<tr><td>10/09/2013</td><td>Rachel, Young Soo, Hao Ding</td></tr>
<tr><td>09/28/2012</td><td>Kshitiz, Daniel & Anna</td></tr>
<tr><td>10/16/2013</td><td>Jesse,& Justin D.</td></tr>
<tr><td>10/05/2012</td><td>Dr. Kwan-Hwi Lee & Nicole</td></tr>
<tr><td>10/23/2013</td><td>Terry Lee, Oscar, & Justin Lee</td></tr>
<tr><td>10/12/2012</td><td>Hyok, Katya & Nicholas</td></tr>
<tr><td>10/30/2013</td><td>Kshitiz & Peter</td></tr>
<tr><td>10/19/2012</td><td>Jesse & Jinah</td></tr>
<tr><td>11/06/2013</td><td>Sam & Alex</td></tr>
<tr><td>10/24/2012</td><td>Ki-Hwan & Peter</td></tr>
<tr><td>11/13/2013</td><td>Eung-Sam & Paulos</td></tr>
<tr><td>11/1/2012</td><td>Alex, Hee-Seok</td></tr>
<tr><td>11/20/2013</td><td>Jon & Nicole</td></tr>
<tr><td>11/9/2012</td><td>Sam, David</td></tr>
<tr><td>11/25/2013 (Monday)</td><td>Phil & Min Soo</td></tr>
<tr><td>11/16/2012</td><td>Kshitiz, Hyok</td></tr>
<tr><td>12/04/2013</td><td>Daniel Lih & Cameron</td></tr>
<tr><td>11/20/2012</td><td>Katya, Daniel</td></tr>
 
<tr><td>11/30/2012</td><td>Andrew, Jonathan, Anna, Peter</td></tr>
<tr><td>12/7/2012</td><td>Jesse, Jinah</td></tr>
</table>
</table>
<br>
<br>
Line 46: Line 44:


==Lab Jobs==
==Lab Jobs==
[https://docs.google.com/a/uw.edu/document/d/1Xw_gHpacpjQZTpsDJgi-g714FzEZCwjl7xjT4ArenVA/edit SLU undergraduate checklist]
<br><br>
<br><br>
'''Autoclaving'''
[https://docs.google.com/a/uw.edu/document/d/1Rsy0nKSwBWJjQEFaJTBljsj90OISXEWCSlnT1_F3nIA/edit Foege undergraduate checklist]
<br>
 
'''SLU:''' microcentrifuge tubes, glass aspirating pipettes, glassware (as needed)Pack items into autoclave bags, seal with tape and label “Kim Lab 419” Take items down to autoclave facility in basement.
<br>
'''Foege:''' microcentrifuge tubes, glass aspirating pipettes, Water bottles Pack items into autoclave bags, seal with tape. Run autoclave items (apply autoclave tapes) Run autoclave biohazard waste (apply autoclave tapes.)
<br><br>
''' TC Maintenence'''
<br>
'''SLU:''' Fill ethanol supply (dilute 190 proof w/DI water). Refill pipettes in drawers and note remaining supply. Notify Melissa or place order for any materials running low such as pipettes, sterile culture dishes or flasks. Take out biohazard waste to autoclave room (mark “DH Kim 419” and apply autoclave tape). Fold pipette keeper boxes as needed.
<br>
'''Foege:''' Refill pipettes and tubes in drawers and note remaining supply. Fill autoclaved water (approx 2L) of tray in incubator. Fold pipette keeper boxes as needed. Fill ethanol supply (dilute 190 proof w/DI water). Notify Melissa or place order for any materials running low such as pipettes, tubes, dishes, cover glasses, sterile culture dishes or flasks.
<br><br>
''' Stocking and Aliquoting'''
<br>
'''SLU:''' Remove a stock bottle from the freezer and place in 4C overnight to thaw. Check supply remaining and place order for more if needed. Trypsin and Pen Strep should be aliquoted into centrifuge tubes at 10mL, and FBS in 25mL. Label clearly and refreeze.
<br>
'''Foege:''' Trypsin, P/S, FBS<br>
Remove a stock bottle from the freezer and place in 4C overnight to thaw. Check supply remaining and place order for more if needed. Trypsin and Pen Strep should be aliquoted into centrifuge tubes at 10mL, and FBS in 25mL. Label clearly and refreeze.
<br><br>
''' Water Bath'''
<br> Drain water, spray down and wipe with ethanol (be sure to lift bottom plating to clean heating coils). Refill (approx 10L) and add appropriate amount of blue water bath solution.
<br><br>
'''Eye Wash'''<br>
Verify that the stations (by the main door and in TC room at SLU, front sink at Foege) are functioning properly.


==Important Links==
==Important Links==


 
*[http://www.ehs.washington.edu/manuals/lsm/index.shtm UW Laboratory Safety Manual] <br>
* [https://www.google.com/calendar/render?tab=oc Microscope Schedule] <br>
*[http://www.ehs.washington.edu/rbsbiosafe/bsmanualindex.shtm UW Biosafety Manual] <br>
 
*[https://docs.google.com/document/d/1U_71qkVoRCsC45oxgasnQzic21aF3TdBPSjgeOQnBy8/edit Lab contact list] <br>
* [https://docs.google.com/a/uw.edu/spreadsheet/ccc?key=0At43xqZHCbEQdHpweGlrbVo0aktmVE5HNnlQZS1CQmc#gid=0 Order Sheet] <br>
*[https://docs.google.com/document/d/1MScwiw-Z2GkSidB5NMdg__gELezCEg8VLlc86oGZ9_M/edit Lab responsibilities] <br>
*[https://docs.google.com/document/d/1m5A0TNLGMGf_5X9Ld2oXNM2lmE-KsPVXOikyDgQ_9ig/edit#heading=h.gjdgxs Weekly report guidelines] <br>
*[https://www.google.com/calendar/embed?src=jdpaev5ld9e71ua8ehd6bic230%40group.calendar.google.com&ctz=America/Los_Angeles  Kim Lab Calendar] <br>
*[https://docs.google.com/a/uw.edu/spreadsheet/ccc?key=0At43xqZHCbEQdHpweGlrbVo0aktmVE5HNnlQZS1CQmc#gid=0 Order Sheet] <br>
*[https://docs.google.com/spreadsheet/ccc?key=0AlqhPQ3eN3okdG1SOFFjMDVPODJfTkY3elJJT19ocXc#gid=0 Antibody Inventory] <br>
*[https://docs.google.com/spreadsheet/ccc?key=0At43xqZHCbEQdDkybGhWbzRmck5XZ1YzY05vTFNQZGc#gid=2 Liquid Nitrogen Inventory]

Revision as of 13:18, 21 October 2013

HOME        RESEARCH        PEOPLE        PUBLICATIONS        INTERNAL        POSITIONS        NEWS        LINKS        OUTREACH        CONTACT       


Meeting Schedule

Fall Quarter 2013 Group meetings Wednesdays in Foege N403 (3:30pm)

DatePresenters
10/02/2013Cameron
10/09/2013Rachel, Young Soo, Hao Ding
10/16/2013Jesse,& Justin D.
10/23/2013Terry Lee, Oscar, & Justin Lee
10/30/2013Kshitiz & Peter
11/06/2013Sam & Alex
11/13/2013Eung-Sam & Paulos
11/20/2013Jon & Nicole
11/25/2013 (Monday)Phil & Min Soo
12/04/2013Daniel Lih & Cameron


Protocols


Lab Jobs

SLU undergraduate checklist

Foege undergraduate checklist


Important Links

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Kim:Internal&oldid=741458"