Kim:Internal: Difference between revisions

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[[Kim:News | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''NEWS''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:News | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''NEWS''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:Links | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''LINKS''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:Links | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''LINKS''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:Contact | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''CONTACT''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:Outreach | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''OUTREACH''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[[Kim:Contact | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''CONTACT''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
[[Kim:Outreach | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''OUTREACH''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
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==Meeting Schedule==
==Meeting Schedule==
Group meetings in Foege N403 (3:00pm-5:00pm) <br>
Meetings will be held on Wednesdays unless otherwise stated.
 
Journal club presentations are denoted by parentheses.
 
Time: 3:30 PM,
Location: Foege N230 <br>


<table border="1">
[https://docs.google.com/document/d/1BLMoTV66lnZtHVT7WtDoZN8cmag4piagge-TwYEQ7A8/edit <font size = 4> Kim Lab Weekly Meeting Schedules </font>]
<th>Date</th><th>Presenters</th>
<tr><td>09/14/2012</td><td>No meeting</td></tr>
<tr><td>09/21/2012</td><td>Julie, Derek, Cameron & Jungho</td></tr>
<tr><td>09/28/2012</td><td>Kshitiz, Daniel & Anna</td></tr>
<tr><td>10/05/2012</td><td>Dr. Kwan-Hwi Lee & Nicole</td></tr>
<tr><td>10/12/2012</td><td>Hyok, Katya & Nicholas</td></tr>
<tr><td>10/19/2012</td><td>Jesse & Jinah</td></tr>
<tr><td>10/24/2012</td><td>Ki-Hwan & Peter</td></tr>
<tr><td>11/1/2012</td><td>Alex, Hee-Seok</td></tr>
<tr><td>11/9/2012</td><td>Sam, David</td></tr>
<tr><td>11/16/2012</td><td>Kshitiz, Hyok</td></tr>
<tr><td>11/20/2012</td><td>Katya, Daniel</td></tr>
<tr><td>11/30/2012</td><td>Andrew, Jonathan, Anna, Peter</td></tr>
<tr><td>12/7/2012</td><td>Jesse, Jinah</td></tr>
</table>
<br>


==Protocols==
==Protocols==
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==Lab Jobs==
==Lab Jobs==
[https://docs.google.com/a/uw.edu/document/d/1Xw_gHpacpjQZTpsDJgi-g714FzEZCwjl7xjT4ArenVA/edit SLU undergraduate checklist]
<br><br>
<br><br>
'''Autoclaving'''
[https://docs.google.com/a/uw.edu/document/d/1Rsy0nKSwBWJjQEFaJTBljsj90OISXEWCSlnT1_F3nIA/edit Foege undergraduate checklist]
<br>
 
'''SLU:''' microcentrifuge tubes, glass aspirating pipettes, glassware (as needed)Pack items into autoclave bags, seal with tape and label “Kim Lab 419” Take items down to autoclave facility in basement.
<br>
'''Foege:''' microcentrifuge tubes, glass aspirating pipettes, Water bottles Pack items into autoclave bags, seal with tape. Run autoclave items (apply autoclave tapes) Run autoclave biohazard waste (apply autoclave tapes.)
<br><br>
''' TC Maintenence'''
<br>
'''SLU:''' Fill ethanol supply (dilute 190 proof w/DI water). Refill pipettes in drawers and note remaining supply. Notify Melissa or place order for any materials running low such as pipettes, sterile culture dishes or flasks. Take out biohazard waste to autoclave room (mark “DH Kim 419” and apply autoclave tape). Fold pipette keeper boxes as needed.
<br>
'''Foege:''' Refill pipettes and tubes in drawers and note remaining supply. Fill autoclaved water (approx 2L) of tray in incubator. Fold pipette keeper boxes as needed. Fill ethanol supply (dilute 190 proof w/DI water). Notify Melissa or place order for any materials running low such as pipettes, tubes, dishes, cover glasses, sterile culture dishes or flasks.
<br><br>
''' Stocking and Aliquoting'''
<br>
'''SLU:''' Remove a stock bottle from the freezer and place in 4C overnight to thaw. Check supply remaining and place order for more if needed. Trypsin and Pen Strep should be aliquoted into centrifuge tubes at 10mL, and FBS in 25mL. Label clearly and refreeze.
<br>
'''Foege:''' Trypsin, P/S, FBS<br>
Remove a stock bottle from the freezer and place in 4C overnight to thaw. Check supply remaining and place order for more if needed. Trypsin and Pen Strep should be aliquoted into centrifuge tubes at 10mL, and FBS in 25mL. Label clearly and refreeze.
<br><br>
''' Water Bath'''
<br> Drain water, spray down and wipe with ethanol (be sure to lift bottom plating to clean heating coils). Refill (approx 10L) and add appropriate amount of blue water bath solution.
<br><br>
'''Eye Wash'''<br>
Verify that the stations (by the main door and in TC room at SLU, front sink at Foege) are functioning properly.


==Important Links==
==Important Links==
 
*[https://docs.google.com/file/d/0BytufrsAlJEMOHRULVU0d2hpZ011SG1yXzAxbG5taUhKejVr/edit Washington Nanofabrication Facilities Usage Rates] <br>
 
*[http://www.ehs.washington.edu/manuals/lsm/index.shtm UW Laboratory Safety Manual] <br>
* [https://www.google.com/calendar/render?tab=oc Microscope Schedule] <br>
*[http://www.ehs.washington.edu/rbsbiosafe/bsmanualindex.shtm UW Biosafety Manual] <br>
 
*[https://docs.google.com/document/d/1U_71qkVoRCsC45oxgasnQzic21aF3TdBPSjgeOQnBy8/edit Lab contact list] <br>
* [https://docs.google.com/a/uw.edu/spreadsheet/ccc?key=0At43xqZHCbEQdHpweGlrbVo0aktmVE5HNnlQZS1CQmc#gid=0 Order Sheet] <br>
*[https://docs.google.com/document/d/1XIZC9Q0vlsLBDGbKP4reYEYSSUNqI3slC1G1L2iLtUU/edit New Lab Member Guide] <br>
*[https://docs.google.com/document/d/1MScwiw-Z2GkSidB5NMdg__gELezCEg8VLlc86oGZ9_M/edit Lab responsibilities] <br>
*[https://docs.google.com/document/d/1m5A0TNLGMGf_5X9Ld2oXNM2lmE-KsPVXOikyDgQ_9ig/edit#heading=h.gjdgxs Weekly report guidelines] <br>
*[https://www.google.com/calendar/embed?src=jdpaev5ld9e71ua8ehd6bic230%40group.calendar.google.com&ctz=America/Los_Angeles  Kim Lab Calendar] <br>
*[https://docs.google.com/a/uw.edu/spreadsheet/ccc?key=0At43xqZHCbEQdHpweGlrbVo0aktmVE5HNnlQZS1CQmc#gid=0 Order Sheet] <br>
*[https://docs.google.com/spreadsheet/ccc?key=0AlqhPQ3eN3okdG1SOFFjMDVPODJfTkY3elJJT19ocXc#gid=0 Antibody Inventory] <br>
*[https://docs.google.com/spreadsheet/ccc?key=0At43xqZHCbEQdDkybGhWbzRmck5XZ1YzY05vTFNQZGc#gid=2 Liquid Nitrogen Inventory] <br>
*[https://www.google.com/calendar/render?cid=loevcmp16krbn83f83qr9286co@group.calendar.google.com Foege Microscope Calendar] <br>
*[https://docs.google.com/file/d/0BzdiAZ77Y5tER2FRaHU3aE5oZ0RXSVZLNS00NG1NSHpJdkRr/edit How to Remotely Access the Server]

Revision as of 07:39, 26 November 2014

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