Kim:Internal

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(Lab Jobs)
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==Lab Jobs==
==Lab Jobs==
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<br><br>
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* cleaning <br>
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'''Autoclaving'''
<br>
<br>
 +
'''SLU:''' microcentrifuge tubes, glass aspirating pipettes, glassware (as needed)Pack items into autoclave bags, seal with tape and label “Kim Lab 419” Take items down to autoclave facility in basement.
 +
<br>
 +
'''Foege:''' microcentrifuge tubes, glass aspirating pipettes, Water bottles Pack items into autoclave bags, seal with tape. Run autoclave items (apply autoclave tapes) Run autoclave biohazard waste (apply autoclave tapes.)
 +
<br><br>
 +
''' TC Maintenence'''
 +
<br>
 +
'''SLU:''' Fill ethanol supply (dilute 190 proof w/DI water). Refill pipettes in drawers and note remaining supply. Notify Melissa or place order for any materials running low such as pipettes, sterile culture dishes or flasks. Take out biohazard waste to autoclave room (mark “DH Kim 419” and apply autoclave tape). Fold pipette keeper boxes as needed.
 +
<br>
 +
'''Foege:''' Refill pipettes and tubes in drawers and note remaining supply. Fill autoclaved water (approx 2L) of tray in incubator. Fold pipette keeper boxes as needed. Fill ethanol supply (dilute 190 proof w/DI water). Notify Melissa or place order for any materials running low such as pipettes, tubes, dishes, cover glasses, sterile culture dishes or flasks.
 +
<br><br>
 +
''' Stocking and Aliquoting'''
 +
<br>
 +
'''SLU:''' Remove a stock bottle from the freezer and place in 4C overnight to thaw. Check supply remaining and place order for more if needed. Trypsin and Pen Strep should be aliquoted into centrifuge tubes at 10mL, and FBS in 25mL. Label clearly and refreeze.
 +
<br>
 +
'''Foege:''' Trypsin, P/S, FBS<br>
 +
Remove a stock bottle from the freezer and place in 4C overnight to thaw. Check supply remaining and place order for more if needed. Trypsin and Pen Strep should be aliquoted into centrifuge tubes at 10mL, and FBS in 25mL. Label clearly and refreeze.
 +
<br><br>
 +
''' Water Bath'''
 +
<br> Drain water, spray down and wipe with ethanol (be sure to lift bottom plating to clean heating coils). Refill (approx 10L) and add appropriate amount of blue water bath solution.
 +
<br><br>
 +
'''Eye Wash'''<br>
 +
Verify that the stations (by the main door and in TC room at SLU, front sink at Foege) are functioning properly.
==Important Links==
==Important Links==

Revision as of 13:57, 12 September 2012

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Contents

Meeting Schedule

Group meetings in Foege N403 (3:00pm-5:00pm)

DatePresenters
09/14/2012No meeting
09/21/2012Julie, Derek, Cameron & Jungho
09/28/2012Kshitiz, Daniel & Anna
10/05/2012Dr. Kwan-Hwi Lee & Nicole
10/12/2012Hyok, Katya & Nicholas
10/19/2012Jesse & Jinah
10/24/2012Ki-Hwan & Peter
11/1/2012Alex, Hee-Seok
11/9/2012Sam, David
11/16/2012Kshitiz, Hyok
11/20/2012Katya, Daniel
11/30/2012Andrew, Jonathan, Anna, Peter
12/7/2012Jesse, Jinah


Protocols


Lab Jobs



Autoclaving
SLU: microcentrifuge tubes, glass aspirating pipettes, glassware (as needed)Pack items into autoclave bags, seal with tape and label “Kim Lab 419” Take items down to autoclave facility in basement.
Foege: microcentrifuge tubes, glass aspirating pipettes, Water bottles Pack items into autoclave bags, seal with tape. Run autoclave items (apply autoclave tapes) Run autoclave biohazard waste (apply autoclave tapes.)

TC Maintenence
SLU: Fill ethanol supply (dilute 190 proof w/DI water). Refill pipettes in drawers and note remaining supply. Notify Melissa or place order for any materials running low such as pipettes, sterile culture dishes or flasks. Take out biohazard waste to autoclave room (mark “DH Kim 419” and apply autoclave tape). Fold pipette keeper boxes as needed.
Foege: Refill pipettes and tubes in drawers and note remaining supply. Fill autoclaved water (approx 2L) of tray in incubator. Fold pipette keeper boxes as needed. Fill ethanol supply (dilute 190 proof w/DI water). Notify Melissa or place order for any materials running low such as pipettes, tubes, dishes, cover glasses, sterile culture dishes or flasks.

Stocking and Aliquoting
SLU: Remove a stock bottle from the freezer and place in 4C overnight to thaw. Check supply remaining and place order for more if needed. Trypsin and Pen Strep should be aliquoted into centrifuge tubes at 10mL, and FBS in 25mL. Label clearly and refreeze.
Foege: Trypsin, P/S, FBS
Remove a stock bottle from the freezer and place in 4C overnight to thaw. Check supply remaining and place order for more if needed. Trypsin and Pen Strep should be aliquoted into centrifuge tubes at 10mL, and FBS in 25mL. Label clearly and refreeze.

Water Bath
Drain water, spray down and wipe with ethanol (be sure to lift bottom plating to clean heating coils). Refill (approx 10L) and add appropriate amount of blue water bath solution.

Eye Wash
Verify that the stations (by the main door and in TC room at SLU, front sink at Foege) are functioning properly.

Important Links

Personal tools