Kim:Research: Difference between revisions
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[[Kim | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''HOME''' </font>]] | [[Kim | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''HOME''' </font>]] | ||
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[[Kim:News | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''NEWS''' </font>]] | [[Kim:News | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''NEWS''' </font>]] | ||
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[[Kim:Contact | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''CONTACT''' </font>]] | [[Kim:Contact | <font face="trebuchet ms" size=3 style="color:#ffffff"> '''CONTACT''' </font>]] | ||
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Micro- and nanoengineering | Micro- and nanoengineering for stem cell biology: the promise with a caution (''Trends Biotechnol.'' '''29(8)''', 399-408 [2011])]] | ||
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<font face="trebuchet ms" size=3 style="color:#000">'''Fundamental:</font> <font face="trebuchet ms" size=3 style="color:#00688B"> | <font face="trebuchet ms" size=3 style="color:#000">'''Fundamental:</font> <font face="trebuchet ms" size=3 style="color:#00688B">Mechanics and signaling in the regulation of cell migration and tissue morphogenesis by physical interactions with the extracellular matrix </font> <br> </div> | ||
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Mechanotransduction - from how cells sense mechanical forces in different tissues to how these mechanical forces are transduced into biochemical signals - is an essential biological process in development, normal physiology and disease. In this exciting area, we are particularly interested in investigating the role of mechano-biological processes associated with cell-cell and cell-matrix adhesions (e.g. topography and rigidity of the extracellular matrix) in the regulation of collective and directed cell migration. Using a combination of various techniques, from molecular biology to nanotechnology and live cell imaging, for example, we have been accumulating interesting data suggesting that one of the most important factors distinguishing metastatic from non-metastatic cells could be their ability to collectively invade and migrate towards blood vessels by physically interacting with the surrounding extracellular matrices. By experimenting with the nanotopographically-defined cell adhesion substratum (i.e. quasi 3D cell culture system) and 3D natural/synthetic extracellular matrices, we are investigating the biophysical and signaling mechanisms of collective cell migration driven by the hypothesis that the physical interaction of migrating cells with the surrounding ECM has a crucial role in the collective guidance of cell migration in the context of cancer invasion and wound healing. To test this hypothesis, we recently developed a micro/nanofabricated collective migration assay as an enabling tool for analysis and control of cancer cell invasion and epithelial/endothelial wound healing in a high-throughput, controlled manner. Using these tools, we also explore the potential role of mechanical guidance in the regulation of collective cell migration under the presence/absence of growth factor-induced signals, and test their biomedical implication by screening cytoskeletal and signal transduction pathways. | Mechanotransduction - from how cells sense mechanical forces in different tissues to how these mechanical forces are transduced into biochemical signals - is an essential biological process in development, normal physiology and disease. In this exciting area, we are particularly interested in investigating the role of mechano-biological processes associated with cell-cell and cell-matrix adhesions (e.g. topography and rigidity of the extracellular matrix) in the regulation of collective and directed cell migration and tissue morphogenesis. Using a combination of various techniques, from molecular biology to nanotechnology and live cell imaging, for example, we have been accumulating interesting data suggesting that one of the most important factors distinguishing metastatic from non-metastatic cells could be their ability to collectively invade and migrate towards blood vessels by physically interacting with the surrounding extracellular matrices. By experimenting with the nanotopographically-defined cell adhesion substratum (i.e. quasi 3D cell culture system) and 3D natural/synthetic extracellular matrices, we are investigating the biophysical and signaling mechanisms of collective cell migration driven by the hypothesis that the physical interaction of migrating cells with the surrounding ECM has a crucial role in the collective guidance of cell migration in the context of cancer invasion and wound healing. To test this hypothesis, we recently developed a micro/nanofabricated collective migration assay as an enabling tool for analysis and control of cancer cell invasion and epithelial/endothelial wound healing in a high-throughput, controlled manner. Using these tools, we also explore the potential role of mechanical guidance in the regulation of collective cell migration and tissue morphogenesis under the presence/absence of growth factor-induced signals, and test their biomedical implication by screening cytoskeletal and signal transduction pathways. | ||
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With advances in nanofabrication and biomaterials, scaffolding materials can be designed to integrate biomimetic structural and mechanical cues present in the in vivo ECM environment. Based on ultrastructural analyses of the native heart tissue, we are developing a bio-inspired model cardiac tissue to better understand cardiac tissue structure-function relationships, and to seek applications in stem cell-based therapies for cardiac tissue repair and regeneration. The ultimate goal of this project is to develop nanopatterned functional cardiac patches for treating the | With advances in nanofabrication and biomaterials, scaffolding materials can be designed to integrate biomimetic structural and mechanical cues present in the in vivo ECM environment. Based on ultrastructural analyses of the native heart tissue, we are developing a bio-inspired model cardiac tissue to better understand cardiac tissue structure-function relationships, and to seek applications in stem cell-based therapies for cardiac tissue repair and regeneration. The ultimate goal of this project is to develop nanopatterned functional cardiac patches for treating the damaged heart tissue (e.g. myocardial infarction). The working hypothesis is that cultivation of cardiac cells and/or stem cells on novel biomaterials scaffolds integrated with nanotopographic cues promotes biomimetic anisotropic assembly of uniformly contractile engineered muscle, while simultaneously enabling control over local cell alignment. We further envision that integrating the transplantable stem cells with the proposed nano-grafting techniques have therapeutic potential in repairing cardiac tissue damage and may prevent the onset of heart failure. In order to test these hypotheses, our research focuses on elucidating the relationships between scaffold-mediated nanostructural cues and tissue engineered cardiac graft contractility and function. In addition, the therapeutic potential of a nanopatterned cardiac stem cell graft will be studied in vitro and in vivo (implantation onto the left ventricle in an adult rat model of myocardial infarction). Tissue structure and function will be characterized at various hierarchical scales (molecular, structural, functional) and the obtained experimental data will be used to tailor the conditions and duration of cultivation, leading to engineering implantable grafts. | ||
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Revision as of 03:36, 31 August 2013
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