Kim:Research: Difference between revisions
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<font face="trebuchet ms" size=4 style="color:#000">'''Technological:</font> <font face="trebuchet ms" size=4 style="color:#00688B"> | <font face="trebuchet ms" size=4 style="color:#000">'''Technological:</font> <font face="trebuchet ms" size=4 style="color:#00688B"> biologically inspired, biomimetic materials, devices and systems </font> <br> </div> | ||
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<font face="trebuchet ms" size=4 style="color:#000">'''Fundamental:</font> <font face="trebuchet ms" size=4 style="color:#00688B"> | <font face="trebuchet ms" size=4 style="color:#000">'''Fundamental:</font> <font face="trebuchet ms" size=4 style="color:#00688B"> Cellular mechanobiology and mechanotransduction in engineered tissue models </font> <br> </div> | ||
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[[Image:Translational.tif|thumb|200x200px|Nanoscale cues regulate the structure and function of macroscopic cardiac tissue constructs (''PNAS'' '''107''', 565-570 [2010])]]With advances in nanofabrication and biomaterials, scaffolding materials can be designed to integrate biomimetic structural and mechanical cues present in the in vivo ECM environment. Based on ultrastructural analyses of the native heart tissue, we are developing a bio-inspired model cardiac tissue to better understand cardiac tissue structure-function relationships, and to seek applications in stem cell-based therapies for cardiac tissue repair and regeneration. The ultimate goal of this project is to develop nanopatterned functional cardiac patches for treating the damaged heart tissue (e.g. myocardial infarction). The working hypothesis is that cultivation of cardiac cells and/or stem cells on novel biomaterials scaffolds integrated with nanotopographic cues promotes biomimetic anisotropic assembly of uniformly contractile engineered muscle, while simultaneously enabling control over local cell alignment. We further envision that integrating the transplantable stem cells with the proposed nano-grafting techniques have therapeutic potential in repairing cardiac tissue damage and may prevent the onset of heart failure. In order to test these hypotheses, our research focuses on elucidating the relationships between scaffold-mediated nanostructural cues and tissue engineered cardiac graft contractility and function. In addition, the therapeutic potential of a nanopatterned cardiac stem cell graft will be studied in vitro and in vivo (implantation onto the left ventricle in an adult rat model of myocardial infarction). Tissue structure and function will be characterized at various hierarchical scales (molecular, structural, functional) and the obtained experimental data will be used to tailor the conditions and duration of cultivation, leading to engineering implantable grafts. | [[Image:Translational.tif|thumb|200x200px|Nanoscale cues regulate the structure and function of macroscopic cardiac tissue constructs (''PNAS'' '''107''', 565-570 [2010])]]With advances in nanofabrication and biomaterials, scaffolding materials can be designed to integrate biomimetic structural and mechanical cues present in the in vivo ECM environment. Based on ultrastructural analyses of the native heart tissue, we are developing a bio-inspired model cardiac tissue to better understand cardiac tissue structure-function relationships, and to seek applications in stem cell-based therapies for cardiac tissue repair and regeneration. The ultimate goal of this project is to develop nanopatterned functional cardiac patches for treating the damaged heart tissue (e.g. myocardial infarction). The working hypothesis is that cultivation of cardiac cells and/or stem cells on novel biomaterials scaffolds integrated with nanotopographic cues promotes biomimetic anisotropic assembly of uniformly contractile engineered muscle, while simultaneously enabling control over local cell alignment. We further envision that integrating the transplantable stem cells with the proposed nano-grafting techniques have therapeutic potential in repairing cardiac tissue damage and may prevent the onset of heart failure. In order to test these hypotheses, our research focuses on elucidating the relationships between scaffold-mediated nanostructural cues and tissue engineered cardiac graft contractility and function. In addition, the therapeutic potential of a nanopatterned cardiac stem cell graft will be studied in vitro and in vivo (implantation onto the left ventricle in an adult rat model of myocardial infarction). Tissue structure and function will be characterized at various hierarchical scales (molecular, structural, functional) and the obtained experimental data will be used to tailor the conditions and duration of cultivation, leading to engineering implantable grafts. | ||
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<font size = 3> '''Funding Sources''': | <font size = 3> '''Funding Sources''': | ||
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Revision as of 02:39, 6 April 2014