Klapperich Lab:Hot Press Wire Imprinted Microfluidic Chips: Difference between revisions
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(New page: ==Overview== Fabricate wire-imprinted microfluidic chips on the hot press. ==Materials== *Mechanical Hot Press *Zeonex beads *2 removable nickel plates *4 cm long segments of 0.38 mm wir...) |
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Line 10: | Line 10: | ||
==Procedure== | ==Procedure== | ||
#Set the temperature of the mechanical hot press to | #Set the temperature of the mechanical hot press to 350°F. | ||
#Center a circular arrangement of zeonex beads one layer thick onto one of the metal plates. | #Center a circular arrangement of zeonex beads one layer thick onto one of the metal plates. | ||
#Place the metal plate onto the bottom hot press platen. | #Place the metal plate onto the bottom hot press platen. | ||
#Carefully align the second plate on top of the first plate without disturbing the collection of zeonex beads. | #Carefully align the second plate on top of the first plate without disturbing the collection of zeonex beads. | ||
#Hot press the zeonex for 5 minutes at | #Hot press the zeonex for 5 minutes at 250 psi. | ||
#Remove the metal plates and let them cool enough to be handled. | #Remove the metal plates and let them cool enough to be handled. | ||
#Peel the zeonex disk off the metal plates. | #Peel the zeonex disk off the metal plates. | ||
Line 20: | Line 20: | ||
#Take 0.38 mm wire cut to a length of 2 cm and arrange four of the wire pieces parallel to each other, staggering them by about 1 cm on the zeonex disk. | #Take 0.38 mm wire cut to a length of 2 cm and arrange four of the wire pieces parallel to each other, staggering them by about 1 cm on the zeonex disk. | ||
#Place the metal plate onto the bottom hot press platen. | #Place the metal plate onto the bottom hot press platen. | ||
#Carefully align the second plate on top of the first plate without disturbing the arrangement of wire segments. | #Carefully align the second plate on top of the first plate without disturbing the arrangement of wire segments. | ||
#Hot press the zeonex for 2 minutes at | #Reduce the temperature of the hot press to 320°F. | ||
#Hot press the zeonex for 2 minutes at 500 psi. | |||
#After removing the metal plates, let the plates cool enough to be handled. | #After removing the metal plates, let the plates cool enough to be handled. | ||
#Peel the wire imprinted zeonex disk off of the metal plates. | #Peel the wire imprinted zeonex disk off of the metal plates. | ||
Line 34: | Line 35: | ||
#Set the second metal plate on top of the first plate. | #Set the second metal plate on top of the first plate. | ||
#Preheat the chip by pumping the handle until the hot press platens meet, but the pressure registers 0 psi for 1 minute. | #Preheat the chip by pumping the handle until the hot press platens meet, but the pressure registers 0 psi for 1 minute. | ||
#Bond the base and the imprinted chip by hot pressing for 2 minutes at | #Bond the base and the imprinted chip by hot pressing for 2 minutes at 550 psi. |
Latest revision as of 13:36, 23 January 2008
Overview
Fabricate wire-imprinted microfluidic chips on the hot press.
Materials
- Mechanical Hot Press
- Zeonex beads
- 2 removable nickel plates
- 4 cm long segments of 0.38 mm wire
- Metal tongs
Procedure
- Set the temperature of the mechanical hot press to 350°F.
- Center a circular arrangement of zeonex beads one layer thick onto one of the metal plates.
- Place the metal plate onto the bottom hot press platen.
- Carefully align the second plate on top of the first plate without disturbing the collection of zeonex beads.
- Hot press the zeonex for 5 minutes at 250 psi.
- Remove the metal plates and let them cool enough to be handled.
- Peel the zeonex disk off the metal plates.
- Set the zeonex disk back onto one of the removable metal plates.
- Take 0.38 mm wire cut to a length of 2 cm and arrange four of the wire pieces parallel to each other, staggering them by about 1 cm on the zeonex disk.
- Place the metal plate onto the bottom hot press platen.
- Carefully align the second plate on top of the first plate without disturbing the arrangement of wire segments.
- Reduce the temperature of the hot press to 320°F.
- Hot press the zeonex for 2 minutes at 500 psi.
- After removing the metal plates, let the plates cool enough to be handled.
- Peel the wire imprinted zeonex disk off of the metal plates.
- Pop the wire pieces out of the zeonex disk.
- Drill, from the grooved side, a hole at each end of an imprinted channel so that the hole overlaps the channel slightly.
- Using tweezers, remove any residual plastic from around the drilled hole.
- Bonding
- Set the temperature of the hot press to 276°F.
- Place an imprinted chip on top of a microfluidic chip base with the grooved side facing the chip base.
- Place the stack onto one of the removable metal plates, and set the metal
plate on the bottom platen.
- Set the second metal plate on top of the first plate.
- Preheat the chip by pumping the handle until the hot press platens meet, but the pressure registers 0 psi for 1 minute.
- Bond the base and the imprinted chip by hot pressing for 2 minutes at 550 psi.