Klapperich Lab:Notebook/Lab Meeting Notes/2008/09/23: Difference between revisions

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† Flu R01 <br>
† Flu R01 <br>
* Meeting on 9/12<br>
* Meeting on 9/12<br>
*1. We are not changing the sample collection procedures at this time.<br>
2. We are aiming to get 200 samples this season, starting when flu hits North America as recorded by CDC website.<br>
3. We may collect samples from anther ER location on BUMC campus.<br>
4. We are going to hire a courier to transport the samples on scheduled days!!!<br>
5. They want to know what "confirmed positive" samples we have from anti-sera assays (and we do, too), but I told them we are doing the plaque assay first and will move on to the anti-sera for a subset of the samples that were cytopathic positive starting likely in Nov.<br>
6. We are still going to split the VTM in our hood and take it over there. <br>
* Plaque assay. <br>
* Plaque assay. <br>


Revision as of 07:53, 23 September 2008

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23 September 2008 Lab Meeting Agenda

† Announcements

  • Fall Group Meetings will be on Tuesdays from 12-2pm in room 705
  • Register for CIMIT conference if you want. Irene can reimburse.
  • Register for MRS Boston if you want to.
  • Room 720 needs to be kept clean.
  • Supply costs are out of control! Conserve.

† Flu R01

  • Meeting on 9/12
  • 1. We are not changing the sample collection procedures at this time.

2. We are aiming to get 200 samples this season, starting when flu hits North America as recorded by CDC website.
3. We may collect samples from anther ER location on BUMC campus.
4. We are going to hire a courier to transport the samples on scheduled days!!!
5. They want to know what "confirmed positive" samples we have from anti-sera assays (and we do, too), but I told them we are doing the plaque assay first and will move on to the anti-sera for a subset of the samples that were cytopathic positive starting likely in Nov.
6. We are still going to split the VTM in our hood and take it over there.

  • Plaque assay.

† SEPSIS Project/Chip Bonding Issues

  • What is the STD temperature and pressure for bonding??? We need to nail this down.
  • What material are the plaques made out of? We should try to get resin that is the same material as the plaques that we bought (JP)
  • We can think about building our own straw system like Fraunhofer's.

†RNA project


  • Sonali will teach Stephanie Jurkat Culture.

† COBRA

  • Mark finish control circuit (Temp, humidity, pressure) design and ordered parts
  • Evaporation fixture will be picked up during this week, and assembled after comeback
  • HELEN says: Jaephil should take a representative sample of what Alexis proposed to be your input: 100 uL of DI water with bacteria (MSSA should be our bacteria)

And demonstrate ability to deliver the specified amount from the microfluidics.

  • Tests on chips with covers underway
  • Jane is investigating the multilayer design

† Valve Array

  • Waiting for fixture still from Melissa


† Fraunhofer: LOAC

  • Still Still troubleshooting

† Biointerfaces group

  • Brett, Mincheol, Jaephil and Hussam had meeting and decided to try DRIE (by Brett) with SU-8 (by Mincheol) together
  • i-line(365nm)has diffraction limiting resolution about 2um at best
  • Mincheol will try the device as we have it
  • Hussam, Brett and MinCheol are going to make a protocol for this process
  • AZP 4620 from Clariant. Hussam will try this photoresist (positive)

† CIMIT Flu

  • Met with Sepsis Nurse Tuesday. Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09

† PCR

  • QQ Model has 30 cycles. MinCheol will try the model. Need to upgrade the Star-CD program. Dan Kamalic has to do this.
  • Dan fixed Star-CD.
  • What are the specs on Wong's pump? More clamps for the syringe. 2 clamps, the secured syringe does not move. It can be set to 4 decimal places for the speed. Harvard Apparatus PHD2000 programmable.
  • PHD2000.ordered very expensive....respect it!
  • QQ needs to design a TaqMan Assay for the lambda phage PCR
  • PEG results are good but we need to get rid of the primer-dimers before publication


† RCA

  • STILL Working on a test chip to examine mat'ls compatibility
  • 50 microliter test chip made/ may be and issue getting the stuff out. Especially after the wash.
  • Heiko, Sonali, and Jaephil write their own part in a whitepaper and forward it to Cathie by 14th.

† Senior project

  • New students will be recruited.
  • Megan works on micro membrane valve and pump development as sr project.

† F31: Cochlea

† Silica Optimization (Lambda):

  • Ongoing


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