Klapperich Lab:Notebook/Lab Meeting Notes/2008/09/23: Difference between revisions

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6. We are still going to split the VTM in our hood and take it over there. <br>
6. We are still going to split the VTM in our hood and take it over there. <br>
* Plaque assay. <br>
* Plaque assay. <br>
* Cathie has to submit the progress report to INSPIR. <br>
<br>
<br>
† SEPSIS Project/'''Chip Bonding Issues''' <br>
† SEPSIS Project/'''Chip Bonding Issues''' <br>

Revision as of 08:07, 23 September 2008

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23 September 2008 Lab Meeting Agenda

† Jaephil's research report.

† Announcements

  • Fall Group Meetings will be on Tuesdays from 12-2pm in room 705
  • Register for CIMIT conference if you want. Irene can reimburse.
  • Register for MRS Boston if you want to.
  • Room 720 needs to be kept clean.
  • Supply costs are out of control! Conserve.


† Flu R01

  • Meeting on 9/12

1. We are not changing the sample collection procedures at this time.
2. We are aiming to get 200 samples this season, starting when flu hits North America as recorded by CDC website.
3. We may collect samples from anther ER location on BUMC campus.
4. We are going to hire a courier to transport the samples on scheduled days!!!
5. They want to know what "confirmed positive" samples we have from anti-sera assays (and we do, too), but I told them we are doing the plaque assay first and will move on to the anti-sera for a subset of the samples that were cytopathic positive starting likely in Nov.
6. We are still going to split the VTM in our hood and take it over there.

  • Plaque assay.
  • Cathie has to submit the progress report to INSPIR.


† SEPSIS Project/Chip Bonding Issues

  • What is the STD temperature and pressure for bonding??? We need to nail this down.
  • What material are the plaques made out of? We should try to get resin that is the same material as the plaques that we bought (JP)
  • We can think about building our own straw system like Fraunhofer's.
  • Sonali finished EF data in blood
  • B Sub data in blood?
  • E coli in pee/blood?
  • Hire UG student to help?


†RNA project

  • Sonali will teach Stephanie Jurkat Culture.
  • Jeff Braman will visit in early Dec.

† COBRA

  • Mark finish control circuit (Temp, humidity, pressure) design and ordered parts
  • Evaporation fixture will be picked up during this week, and assembled after comeback
  • HELEN says: Jaephil should take a representative sample of what Alexis proposed to be your input: 100 uL of DI water with bacteria (MSSA should be our bacteria)

And demonstrate ability to deliver the specified amount from the microfluidics.

  • Tests on chips with covers underway
  • Jane is investigating the multilayer design


† Valve Array/Valve building (FJ)

  • Waiting for fixture still from Melissa
  • Got drawings and preliminary info from Pennarthur group at UCSB.
  • Waiting for further contact.


† Fraunhofer: LOAC

  • Still Still troubleshooting


† Biointerfaces group

  • Brett, Mincheol, Jaephil and Hussam had meeting and decided to try DRIE (by Brett) with SU-8 (by Mincheol) together
  • i-line(365nm)has diffraction limiting resolution about 2um at best
  • Mincheol will try the device as we have it
  • Preliminary bead trapping DEMONSTRATED.
  • Hussam, Brett and MinCheol are going to make a protocol for this process
  • AZP 4620 from Clariant. Hussam will try this photoresist (positive)


† CIMIT Flu

  • Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09


† PCR

  • QQ Model has 30 cycles. MinCheol will try the model. Need to upgrade the Star-CD program. Dan Kamalic has to do this.
  • PHD2000.ordered very expensive....respect it!
  • QQ designed a TaqMan Assay for the lambda phage PCR
  • PEG results are good but we need to get rid of the primer-dimers before publication -- update "primer-dimer" features appear in the pre cycled gel lanes. Why??


† RCA

  • Cathie Working on Whitepaper


† Senior project

  • New student recruited.
  • Megan works on micro membrane valve and pump development as sr project.


† F31: Cochlea

† Silica Optimization (Lambda):

  • Ongoing


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