Klapperich Lab:Notebook/Lab Meeting Notes/2008/09/23: Difference between revisions
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* DO NOT CHANGE THE PROTOCOL W/OUT TALKING TO ME OR JAEPHIL<br> | * DO NOT CHANGE THE PROTOCOL W/OUT TALKING TO ME OR JAEPHIL<br> | ||
* Fixture for Sonali (HM).<br> | * Fixture for Sonali (HM).<br> | ||
* Buy Ticona COC materials (JD). <br> | |||
* Sonali finished EF data in blood <br> | * Sonali finished EF data in blood. <br> | ||
* B Sub data in blood | * B Sub data in blood extracted redo PCR/contaminated water. <br> | ||
* E coli in pee/blood? <br> | * PCR Pipettes must buy...no one uses them for non-PCR use!!!!!!!! <br> | ||
* SEM image analysis of pore size? <br> | * E coli in pee/blood? This week. <br> | ||
* Analytical calculations for pore size? D'arcy's law is off by orders of magnitude. Next steps? MinCheol's model | * SEM image analysis of pore size? - We have images. We need to do thresholding, and avg. pore size measurement. Jessica's MATLAB Code. Send her code to JANE to look at. <br> | ||
* Analytical calculations for pore size? D'arcy's law is off by orders of magnitude. Next steps? MinCheol's model (randomly generated monolith) Min-Cheol and Jane working on it.<br> | |||
<br> | <br> | ||
†RNA project | †RNA project | ||
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* Jeff Braman will visit in early Dec. <br> | * Jeff Braman will visit in early Dec. <br> | ||
* We can think about building our own straw system like Fraunhofer's.<br> | * We can think about building our own straw system like Fraunhofer's.<br> | ||
* Discuss Anirban's protocol and system etc. What gets shared. What gets Used. (Cathie)<br> | |||
<br> | <br> | ||
† COBRA <br> | † COBRA <br> | ||
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* HELEN says: Jaephil should take a representative sample of what Alexis proposed to be your input: 100 uL of DI water with bacteria (MSSA should be our bacteria) | * HELEN says: Jaephil should take a representative sample of what Alexis proposed to be your input: 100 uL of DI water with bacteria (MSSA should be our bacteria) | ||
And demonstrate ability to deliver the specified amount from the microfluidics.<br> | And demonstrate ability to deliver the specified amount from the microfluidics.<br> | ||
* Jane working on CIMIT fellowship presentation and future plans for THESIS! <br> | * Jane working on CIMIT fellowship presentation and future plans for THESIS! <br> | ||
<br> | <br> | ||
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* Got drawings and preliminary info from Pennarthur group at UCSB.<br> | * Got drawings and preliminary info from Pennarthur group at UCSB.<br> | ||
* Waiting for further contact.<br> | * Waiting for further contact.<br> | ||
* Update: Monday 29 Sept Frank contacted Shane Conran of Superior Fluid Solutions (referred by Jess from UCSB). Will draw up preliminary design and send to us for evaluation.<br> | |||
<br> | <br> | ||
† Fraunhofer: LOAC <br> | † Fraunhofer: LOAC <br> | ||
* Still Still troubleshooting <br> | * Still Still Still troubleshooting <br> | ||
<br> | <br> | ||
† Biointerfaces group <br> | † Biointerfaces group <br> | ||
* Preliminary bead trapping DEMONSTRATED. <br> | * Preliminary bead trapping DEMONSTRATED. <br> | ||
* | * Mask writer down. Make a CR mask on Friday.<br> | ||
* | * Make a mold for Brett next week. <br> | ||
* Do preliminary E. coli experiment soon. <br> | |||
<br> | <br> | ||
† CIMIT | † CIMIT- Colson Grant<br> | ||
* Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09<br> | * Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09<br> | ||
* STAR-CD Renewal | * STAR-CD Renewal - January ($2K)<br> | ||
<br> | <br> | ||
† PCR <br> | † PCR <br> | ||
* QQ Model has 30 cycles. MinCheol | * QQ Model has 30 cycles. MinCheol made the computational model. Star-CD program has been upgraded by Dan Kamalic. <br> | ||
* PHD2000.ordered very expensive....respect it!<br> | * PHD2000.ordered very expensive....respect it!<br> | ||
* QQ designed a TaqMan Assay for the lambda phage PCR <br> | * QQ designed a TaqMan Assay for the lambda phage PCR <br> | ||
* PEG results are good but we need to get rid of the primer-dimers before publication -- update "primer-dimer" features appear in the pre cycled gel lanes. Why??<br> | * PEG results are good but we need to get rid of the primer-dimers before publication -- update "primer-dimer" features appear in the pre cycled gel lanes. Why?? Not primer dimers, contamination. (SM will consult)<br> | ||
* Contamination. Make an internal control on the chip. <br> | |||
* What is the time consuming step on using a new chip every time? (QQ)<br> | |||
* MinCheol is making three simple PCR models to optimize temperature distribution along to the channel. <br> | |||
<br> | <br> | ||
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<br> | <br> | ||
† Senior project <br> | † Senior project <br> | ||
* New student recruited. <br> | * New student recruited. Theodore. <br> | ||
* Megan works on micro membrane valve and pump development as sr project. <br> | * Megan works on micro membrane valve and pump development as sr project. <br> | ||
<br> | <br> | ||
† F31: Cochlea <br> | † F31: Cochlea <br> | ||
* ARO Abstract due October 1st. <br> | |||
* Raw data late this week. <br> | |||
* | |||
<br> | <br> | ||
† Silica Optimization (Lambda): <br> | † Silica Optimization (Lambda): <br> | ||
* Ongoing <br> | * Ongoing <br> | ||
[http://openwetware.org/wiki/Image:Progress_9-15_to_9-21_.doc hussam's Update] <br> | |||
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Revision as of 11:35, 29 September 2008
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23 September 2008 Lab Meeting Agenda† Jaephil's research report. † Announcements
1. We are not changing the sample collection procedures at this time.
6. We are still going to split the VTM in our hood and take it over there.
† COBRA
And demonstrate ability to deliver the specified amount from the microfluidics.
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