Klapperich Lab:Notebook/Lab Meeting Notes/2008/09/30

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(30 September 2008 Lab Meeting Agenda)
(30 September 2008 Lab Meeting Agenda)
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<br>
† Biointerfaces group <br>
† Biointerfaces group <br>
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* Make a mold for Brett next week. <br>
+
* Make a mold for Brett this week. <br>
* Do preliminary E. coli experiment soon. <br>
* Do preliminary E. coli experiment soon. <br>
 +
* Making a CR mask Tuesday afternoon. <br>
<br>
<br>
† CIMIT- Colson Grant<br>
† CIMIT- Colson Grant<br>
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* Contamination. Make an internal control on the chip. <br>  
* Contamination. Make an internal control on the chip. <br>  
* '''What is the time consuming step on using a new chip every time? (QQ)<br>'''
* '''What is the time consuming step on using a new chip every time? (QQ)<br>'''
-
* MinCheol is making three simple PCR models to optimize temperature distribution along to the channel. <br>
+
* MinCheol is making Four simple PCR models to optimize temperature distribution along to the channel. <br>
 +
* MinCheol is simulating DNA's Residence Time for simple PCR models. <br>
<br>
<br>

Revision as of 16:13, 29 September 2008

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30 September 2008 Lab Meeting Agenda

† No research report today.

† Announcements

  • A slow migration to 709 can now begin. There will be core equipment entering 720, we will accommodate.
  • Supply costs are out of control! Conserve.


† Flu R01

  • Mark's role outlined this week. He will make fixture for Sonali in near term. Will look at integrated chip in long term.
  • Will do RTPCR and not anti sera assays - Cathie told Chris et al. 9/29.
  • More sample back? Can we put in 1.5 ml saline, collect in 3ml VTM tube directly? Can we amend - Cathie emailed on 9/29.
  • Plaque assay status?
  • Cathie STILL has to submit the progress report to INSPIR.


† SEPSIS Project - CIMIT Bird Flu

  • Toluene method. Why didn't we use it? (QQ, AG)
  • Buy Ticona COC materials (JD).
  • B Sub data in blood extracted redo PCR/contaminated water.
  • PCR Pipettes bought...no one uses them for non-PCR use!!!!!!!!
  • E coli in pee/blood. This week.
  • SEM image analysis of pore size. Jane did preliminary analysis. Can Jane finish this up for the paper?


†RNA project

  • Sonali will teach Stephanie Jurkat Culture.
  • Jeff Braman will visit in early Dec.
  • We have Anirban's protocol and system etc. Issues resolved

† COBRA

  • Mark finish control circuit (Temp, humidity, pressure) design and ordered parts
  • Evaporation data with bacteria or beads?
  • Jane, Cathie, Larry, Ranjith will meet soon to discuss scale up of sol gel in chip process.
  • Jane working on CIMIT fellowship presentation and future plans for THESIS!


† Valve Array/Valve building (FJ)

  • Waiting for fixture still from Melissa
  • Update: Monday 29 Sept Frank contacted Shane Conran of Superior Fluid Solutions (referred by Jess from UCSB). Will draw up preliminary design and send to us for evaluation.


† Fraunhofer: LOAC

  • Still Still Still troubleshooting ??


† Biointerfaces group

  • Make a mold for Brett this week.
  • Do preliminary E. coli experiment soon.
  • Making a CR mask Tuesday afternoon.


† CIMIT- Colson Grant

  • Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
  • STAR-CD Renewal - January ($2K)


† PCR

  • PCR areas defined. Protocols reviewed. Contamination issues discussed....
  • QQ designed a TaqMan Assay for the lambda phage PCR
  • Contamination. Make an internal control on the chip.
  • What is the time consuming step on using a new chip every time? (QQ)
  • MinCheol is making Four simple PCR models to optimize temperature distribution along to the channel.
  • MinCheol is simulating DNA's Residence Time for simple PCR models.


† RCA

  • Cathie Still Working on Whitepaper almost done!


† Senior project


† F31: Cochlea

  • ARO Abstract due October 1st.


† Silica Optimization (Lambda):

  • Ongoing


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