30 September 2008 Lab Meeting Agenda
† No research report today.
† Announcements
- A slow migration to 709 can now begin. There will be core equipment entering 720, we will accommodate.
- Supply costs are out of control! Conserve.
† Flu R01
- Mark's role outlined this week. He will make fixture for Sonali in near term. Will look at integrated chip in long term.
- Will do RTPCR and not anti sera assays - Cathie told Chris et al. 9/29.
- More sample back? Can we put in 1.5 ml saline, collect in 3ml VTM tube directly? What is the issue with doing that? Can we amend - Cathie will look into it with them.
- Plaque assay status. Issue with the overlay.
- Set up Sonali with Jose Re: Overlay advice.
- Cathie has to submit the progress report to INSPIR.
† SEPSIS Project/Chip Bonding Issues
- ZEONEX 690R, Tg, we measured it 141 C
- http://www.zeonex.com/datasheets.asp
- Toluene method. Why didn't we use it? (QQ, AG)
- DO NOT CHANGE THE PROTOCOL W/OUT TALKING TO ME OR JAEPHIL
- Fixture for Sonali (HM).
- Buy Ticona COC materials (JD).
- Sonali finished EF data in blood.
- B Sub data in blood extracted redo PCR/contaminated water.
- PCR Pipettes must buy...no one uses them for non-PCR use!!!!!!!!
- E coli in pee/blood? This week.
- SEM image analysis of pore size? - We have images. We need to do thresholding, and avg. pore size measurement. Jessica's MATLAB Code. Send her code to JANE to look at.
- Analytical calculations for pore size? D'arcy's law is off by orders of magnitude. Next steps? MinCheol's model (randomly generated monolith) Min-Cheol and Jane working on it.
†RNA project
- Sonali will teach Stephanie Jurkat Culture.
- Jeff Braman will visit in early Dec.
- We can think about building our own straw system like Fraunhofer's.
- Discuss Anirban's protocol and system etc. What gets shared. What gets Used. (Cathie)
† COBRA
- Mark finish control circuit (Temp, humidity, pressure) design and ordered parts
- Evaporation fixture will be picked up during this week, and assembled after comeback
- HELEN says: Jaephil should take a representative sample of what Alexis proposed to be your input: 100 uL of DI water with bacteria (MSSA should be our bacteria)
And demonstrate ability to deliver the specified amount from the microfluidics.
- Jane working on CIMIT fellowship presentation and future plans for THESIS!
† Valve Array/Valve building (FJ)
- Waiting for fixture still from Melissa
- Got drawings and preliminary info from Pennarthur group at UCSB.
- Waiting for further contact.
- Update: Monday 29 Sept Frank contacted Shane Conran of Superior Fluid Solutions (referred by Jess from UCSB). Will draw up preliminary design and send to us for evaluation.
† Fraunhofer: LOAC
- Still Still Still troubleshooting
† Biointerfaces group
- Preliminary bead trapping DEMONSTRATED.
- Mask writer down. Make a CR mask on Friday.
- Make a mold for Brett next week.
- Do preliminary E. coli experiment soon.
† CIMIT- Colson Grant
- Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
- STAR-CD Renewal - January ($2K)
† PCR
- QQ Model has 30 cycles. MinCheol made the computational model. Star-CD program has been upgraded by Dan Kamalic.
- PHD2000.ordered very expensive....respect it!
- QQ designed a TaqMan Assay for the lambda phage PCR
- PEG results are good but we need to get rid of the primer-dimers before publication -- update "primer-dimer" features appear in the pre cycled gel lanes. Why?? Not primer dimers, contamination. (SM will consult)
- Contamination. Make an internal control on the chip.
- What is the time consuming step on using a new chip every time? (QQ)
- MinCheol is making three simple PCR models to optimize temperature distribution along to the channel.
† RCA
- Cathie Working on Whitepaper
† Senior project
- New student recruited. Theodore.
- Megan works on micro membrane valve and pump development as sr project.
† F31: Cochlea
- ARO Abstract due October 1st.
- Raw data late this week.
† Silica Optimization (Lambda):
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