Klapperich Lab:Notebook/Lab Meeting Notes/2008/10/14: Difference between revisions

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==14 October 2008 Lab Meeting Agenda==
† MinCheol's research report today.


† Announcements<br>
* JZ and JD out this week at mTAS. <br>
<br>
† Flu R01 <br>
* TO DO More sample back? Can we put in 1.5 ml saline, collect in 3ml VTM tube directly?  YES. Cathie will amend. <br>
* Plaque assay status?<br>
* TO DO Cathie STILL STILL has to submit the progress report to INSPIR. <br>
<br>
† SEPSIS Project - CIMIT Bird Flu <br>
* B sub in blood is done. Lets talk about comepleting the gram positive plots so they "match." <br>
* SEM image analysis of pore size. DONE. Thanks JZ<br>
* Run E. coli RT-PCR using primers for a genomic target.<br>
* Report DNA amounts along with the RT-PCR data for the Sepsis paper.<br>
<br>
†RNA project
* Jeff Braman will visit in early Dec. <br>
*  <br>
* Do dilutions. Send to Bioanalyzer. <br>
<br>
† COBRA <br>
* Mark finish control circuit <br>
* Fluorescence microscopy available from 720. Try beads experiment. DONE. <br>
* Bacteria Experiment? <br>
* Jane, Cathie, Larry, Ranjith met Friday. JZ will try process with modified washes.  <br>
<br>
† Valve Array/Valve building (FJ) <br>
* Waiting for fixture still from Melissa. Hopefully this week. UPDATE? <br>
* 9 Oct Shane says schematics are completed and would be emailed "right away".  Have not received anything.  Will contact 14 Oct to figure out why.<br>
<br>
† Fraunhofer: LOAC <br>
* Update? <br>
* Alex working on more toluene bonding issues. <br>
* Try Ticona material. (HM, JD, AG) <br>
* Still Still Still troubleshooting '''??'''<br>
<br>
† Biointerfaces group <br>
* Mold still not made. Mask potentially Wed AM. When mask is made. <br>
* Do preliminary E. coli experiment soon. Sonali will train. <br>
<br>
† CIMIT- Colson Grant<br>
* Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09<br>
* STAR-CD Renewal - January ($2K)<br>
<br>
† PCR <br>
* <br>
* QQ will run it on a gel to check. <br>
* '''Run Gels for water Wash steps - show me and Sonali''' UPDATE? <br>
* Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - Will try after fixes and report next week. UPDATE? <br>
'''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. <br>'''
* Qingqing has to do experiments with MinCheol's designs. AS SOON AS CLEANING CONTEROLS ARE IN PLACE. <br>
<br>
† RCA <br>
* Cathie Still Still Working on Whitepaper. <br>
<br>
† Senior project <br>
* Megan ordering oligos and primers for the RCA. Quote. Check with Heiko on his sources. <br>
* Haven't seen Teddy yet. What is up with him? <br>
<br>
† F31: Cochlea <br>
*
* <br>
<br>
† Silica Optimization (Lambda): <br>
* Ongoing. More concentrations. . <br>
* Pressure testing on these channels when they are flowing. <br>
[http://openwetware.org/wiki/Image:Progress_10-6_to_10-12.doc  hussam's update ] <br>


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Revision as of 09:01, 14 October 2008

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14 October 2008 Lab Meeting Agenda

† MinCheol's research report today.

† Announcements

  • JZ and JD out this week at mTAS.


† Flu R01

  • TO DO More sample back? Can we put in 1.5 ml saline, collect in 3ml VTM tube directly? YES. Cathie will amend.
  • Plaque assay status?
  • TO DO Cathie STILL STILL has to submit the progress report to INSPIR.


† SEPSIS Project - CIMIT Bird Flu

  • B sub in blood is done. Lets talk about comepleting the gram positive plots so they "match."
  • SEM image analysis of pore size. DONE. Thanks JZ
  • Run E. coli RT-PCR using primers for a genomic target.
  • Report DNA amounts along with the RT-PCR data for the Sepsis paper.


†RNA project

  • Jeff Braman will visit in early Dec.

  • Do dilutions. Send to Bioanalyzer.

† COBRA

  • Mark finish control circuit
  • Fluorescence microscopy available from 720. Try beads experiment. DONE.
  • Bacteria Experiment?
  • Jane, Cathie, Larry, Ranjith met Friday. JZ will try process with modified washes.


† Valve Array/Valve building (FJ)

  • Waiting for fixture still from Melissa. Hopefully this week. UPDATE?
  • 9 Oct Shane says schematics are completed and would be emailed "right away". Have not received anything. Will contact 14 Oct to figure out why.


† Fraunhofer: LOAC

  • Update?
  • Alex working on more toluene bonding issues.
  • Try Ticona material. (HM, JD, AG)
  • Still Still Still troubleshooting ??


† Biointerfaces group

  • Mold still not made. Mask potentially Wed AM. When mask is made.
  • Do preliminary E. coli experiment soon. Sonali will train.


† CIMIT- Colson Grant

  • Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
  • STAR-CD Renewal - January ($2K)


† PCR


  • QQ will run it on a gel to check.
  • Run Gels for water Wash steps - show me and Sonali UPDATE?
  • Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - Will try after fixes and report next week. UPDATE?

* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture.

  • Qingqing has to do experiments with MinCheol's designs. AS SOON AS CLEANING CONTEROLS ARE IN PLACE.


† RCA

  • Cathie Still Still Working on Whitepaper.


† Senior project

  • Megan ordering oligos and primers for the RCA. Quote. Check with Heiko on his sources.
  • Haven't seen Teddy yet. What is up with him?


† F31: Cochlea



† Silica Optimization (Lambda):

  • Ongoing. More concentrations. .
  • Pressure testing on these channels when they are flowing.



hussam's update


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