Klapperich Lab:Notebook/Lab Meeting Notes/2008/10/14

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(14 October 2008 Lab Meeting Agenda)
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* Pressure testing on these channels when they are flowing. <br>
* Pressure testing on these channels when they are flowing. <br>
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[http://openwetware.org/wiki/Image:Progress_10-6_to_10-12.doc  hussam's update ] <br>
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Revision as of 12:01, 14 October 2008

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14 October 2008 Lab Meeting Agenda

† MinCheol's research report today.

† Announcements

  • JZ and JD out this week at mTAS.


† Flu R01

  • TO DO More sample back? Can we put in 1.5 ml saline, collect in 3ml VTM tube directly? YES. Cathie will amend.
  • Plaque assay status?
  • TO DO Cathie STILL STILL has to submit the progress report to INSPIR.


† SEPSIS Project - CIMIT Bird Flu

  • B sub in blood is done. Lets talk about comepleting the gram positive plots so they "match."
  • SEM image analysis of pore size. DONE. Thanks JZ
  • Run E. coli RT-PCR using primers for a genomic target.
  • Report DNA amounts along with the RT-PCR data for the Sepsis paper.


†RNA project

  • Jeff Braman will visit in early Dec.

  • Do dilutions. Send to Bioanalyzer.

† COBRA

  • Mark finish control circuit
  • Fluorescence microscopy available from 720. Try beads experiment. DONE.
  • Bacteria Experiment?
  • Jane, Cathie, Larry, Ranjith met Friday. JZ will try process with modified washes.


† Valve Array/Valve building (FJ)

  • Waiting for fixture still from Melissa. Hopefully this week. UPDATE?
  • 9 Oct Shane says schematics are completed and would be emailed "right away". Have not received anything. Will contact 14 Oct to figure out why.


† Fraunhofer: LOAC

  • Update?
  • Alex working on more toluene bonding issues.
  • Try Ticona material. (HM, JD, AG)
  • Still Still Still troubleshooting ??


† Biointerfaces group

  • Mold still not made. Mask potentially Wed AM. When mask is made.
  • Do preliminary E. coli experiment soon. Sonali will train.


† CIMIT- Colson Grant

  • Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
  • STAR-CD Renewal - January ($2K)


† PCR


  • QQ will run it on a gel to check.
  • Run Gels for water Wash steps - show me and Sonali UPDATE?
  • Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - Will try after fixes and report next week. UPDATE?

* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture.

  • Qingqing has to do experiments with MinCheol's designs. AS SOON AS CLEANING CONTEROLS ARE IN PLACE.


† RCA

  • Cathie Still Still Working on Whitepaper.


† Senior project

  • Megan ordering oligos and primers for the RCA. Quote. Check with Heiko on his sources.
  • Haven't seen Teddy yet. What is up with him?


† F31: Cochlea



† Silica Optimization (Lambda):

  • Ongoing. More concentrations. .
  • Pressure testing on these channels when they are flowing.

hussam's update


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