Klapperich Lab:Notebook/Lab Meeting Notes/2008/11/04: Difference between revisions

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† PCR <br>
† PCR <br>
* <br>
* QQ gel issues. Resolved?? <br>
* QQ gel issues. Resolved  <br>
* Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - Will try after fixes and report next week. UPDATE? <br>
* Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - Will try after fixes and report next week. UPDATE? <br>
'''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. <br>'''
'''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. <br>'''

Revision as of 19:24, 3 November 2008

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4 November 2008 Lab Meeting Agenda

† Mark's research report today.

† Announcements

  • 720 Migration in full swing. Make sure things you do not want "shared" are clearly marked. Check with Sonali before using any space in 709.


† Flu R01

  • No amendment needed. Flu meeting on 10/31 to discuss new procedures.
  • Plaque assay status?
  • Cathie Submitted the progress report to INSPIR.


† SEPSIS Project - CIMIT Bird Flu

  • Run E. coli RT-PCR using primers for a genomic target.
  • Report DNA amounts along with the RT-PCR data for the Sepsis paper.


†RNA project

  • Jeff Braman will visit in early Dec.
  • Do dilutions. Send to Bioanalyzer.

† COBRA

  • Mark finish control circuit
  • Bacteria Experiment. Failed first time. Any progress?
  • Jane, Cathie, Larry, Ranjith met again. JZ will try process with teflon substrates.


† Valve Array/Valve building (FJ)

  • Waiting for fixture still from Melissa. Hopefully this week. UPDATE?
  • 9 Oct Shane says schematics are completed and would be emailed "right away". Have not received anything. Will contact 14 Oct to figure out why.


† Fraunhofer: LOAC

  • Update?
  • Alex working on more toluene bonding issues.
  • Try Ticona material. (HM, JD, AG)
  • Still Still Still troubleshooting ??


† Biointerfaces group

  • Mold still not made. Mask is made.
  • Do preliminary E. coli experiment soon. Sonali will train.


† CIMIT- Colson Grant

  • Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
  • STAR-CD Renewal - January ($2K)


† PCR

  • QQ gel issues. Resolved??
  • Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - Will try after fixes and report next week. UPDATE?

* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture.

  • Qingqing has to do experiments with MinCheol's designs. AS SOON AS CLEANING CONTEROLS ARE IN PLACE.


† RCA

  • Cathie submitted One pager Whitepaper. Waiting for response.


† Senior project

  • Megan ordering oligos and primers for the RCA. Quote. Check with Heiko on his sources.
  • Haven't seen Teddy yet. What is up with him?


† F31: Cochlea



† Silica Optimization (Lambda):

  • Ongoing. More concentrations. .
  • Pressure testing on these channels when they are flowing.



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