Klapperich Lab:Notebook/Lab Meeting Notes/2008/11/04: Difference between revisions

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† Announcements<br>
† Announcements<br>
* 720 Migration in full swing. Make sure things you do not want "shared" are clearly marked.  Check with Sonali before using any space in 709. <br>
* 720 Migration in full swing. Make sure things you do not want "shared" are clearly marked.  Check with Sonali before using any space in 709. <br>
* ALL - please heed lab safety rules. Re-read Sonali's email of earlier this week!<br>
<br>
<br>
† Flu R01 <br>
† Flu R01 <br>
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† Valve Array/Valve building (FJ) <br>
† Valve Array/Valve building (FJ) <br>
* Waiting for fixture still from Melissa. Hopefully this week. UPDATE? <br>
* Waiting for fixture still from Melissa. Hopefully this week. UPDATE? <br>
* 9 Oct Shane says schematics are completed and would be emailed "right away".  Have not received anything.  Will contact 14 Oct to figure out why.<br>
* Received schematic and quote.  Frank met with Dr. Do to go over schematic and see what's next.  Met with Sonali (3 Nov) to better delineate system requirements, will meet with Mark to coordinate fixture/pressure-driven flow system interfacing before submitting revised specs to Superior Fluid Solutions.<br>
<br>
<br>
† Fraunhofer: LOAC <br>
† Fraunhofer: LOAC <br>
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<br>
<br>
† Biointerfaces group <br>
† Biointerfaces group <br>
* Mold still not made. Mask is made. <br>
* Mask writer's calibration was done. <br>
* Do preliminary E. coli experiment soon. Sonali will train. <br>
* Paul Mak told that Mask aligner at Photonics center has UV filter. Try that machine.<br>
* Preliminary E. coli experiment is ongoing. <br>
* Try DAPI bacteria stain to visualize e-coli soon. <br>
<br>
<br>
† CIMIT- Colson Grant<br>
† CIMIT- Colson Grant<br>
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'''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. <br>'''
'''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. <br>'''
* Qingqing has to do experiments with MinCheol's designs. AS SOON AS CLEANING CONTEROLS ARE IN PLACE. <br>
* Qingqing has to do experiments with MinCheol's designs. AS SOON AS CLEANING CONTEROLS ARE IN PLACE. <br>
* Mincheol will work on DNA migration simulation after IMECE 2008.<br>
<br>
<br>
† RCA <br>
† RCA <br>
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<br>
† F31: Cochlea <br>
† F31: Cochlea <br>
*
* Received paper edits from Doug.<br>
* <br>
* Make changes and give to Cathie ASAP. <br>
* Journal chosen? <br>
* Need to order Alpha-Tectorin protein.<br>
<br>
<br>
† Silica Optimization (Lambda): <br>
† Silica Optimization (Lambda): <br>

Revision as of 08:16, 4 November 2008

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4 November 2008 Lab Meeting Agenda

† Mark's research report today.

† Announcements

  • 720 Migration in full swing. Make sure things you do not want "shared" are clearly marked. Check with Sonali before using any space in 709.
  • ALL - please heed lab safety rules. Re-read Sonali's email of earlier this week!


† Flu R01

  • No amendment needed. Flu meeting on 10/31 to discuss new procedures.
  • Plaque assay status?
  • Cathie Submitted the progress report to INSPIR.


† SEPSIS Project - CIMIT Bird Flu

  • Run E. coli RT-PCR using primers for a genomic target.
  • Report DNA amounts along with the RT-PCR data for the Sepsis paper.


†RNA project

  • Jeff Braman will visit in early Dec.
  • Do dilutions. Send to Bioanalyzer.

† COBRA

  • Mark finish control circuit
  • Bacteria Experiment. Failed first time. Any progress?
  • Jane, Cathie, Larry, Ranjith met again. JZ will try process with teflon substrates.


† Valve Array/Valve building (FJ)

  • Waiting for fixture still from Melissa. Hopefully this week. UPDATE?
  • Received schematic and quote. Frank met with Dr. Do to go over schematic and see what's next. Met with Sonali (3 Nov) to better delineate system requirements, will meet with Mark to coordinate fixture/pressure-driven flow system interfacing before submitting revised specs to Superior Fluid Solutions.


† Fraunhofer: LOAC

  • Update?
  • Alex working on more toluene bonding issues.
  • Try Ticona material. (HM, JD, AG)
  • Still Still Still troubleshooting ??


† Biointerfaces group

  • Mask writer's calibration was done.
  • Paul Mak told that Mask aligner at Photonics center has UV filter. Try that machine.
  • Preliminary E. coli experiment is ongoing.
  • Try DAPI bacteria stain to visualize e-coli soon.


† CIMIT- Colson Grant

  • Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
  • STAR-CD Renewal - January ($2K)


† PCR

  • QQ gel issues. Resolved??
  • Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - Will try after fixes and report next week. UPDATE?

* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture.

  • Qingqing has to do experiments with MinCheol's designs. AS SOON AS CLEANING CONTEROLS ARE IN PLACE.
  • Mincheol will work on DNA migration simulation after IMECE 2008.


† RCA

  • Cathie submitted One pager Whitepaper. Waiting for response.


† Senior project

  • Megan ordering oligos and primers for the RCA. Quote. Check with Heiko on his sources.
  • Haven't seen Teddy yet. What is up with him?


† F31: Cochlea

  • Received paper edits from Doug.
  • Make changes and give to Cathie ASAP.
  • Journal chosen?
  • Need to order Alpha-Tectorin protein.


† Silica Optimization (Lambda):

  • Ongoing. More concentrations. .
  • Pressure testing on these channels when they are flowing.



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