Klapperich Lab:Notebook/Lab Meeting Notes/2008/11/11: Difference between revisions

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† Flu R01 <br>
† Flu R01 <br>
* Courier service! Sample transport set up. <br>
* Courier service! Sample transport set up. <br>
* Plaque assay status?<br>
* Plaque assay status -- plaques and everything!Yeah! <br>
* Mark - status of fixture? <br>
* Mark - status of fixture? <br>
<br>
<br>
Line 22: Line 22:
†RNA project
†RNA project
* Jeff Braman will visit in early Dec. <br>
* Jeff Braman will visit in early Dec. <br>
* Do cDNA prep. Spec, gels, send to Jeff B. <br>
* Do cDNA prep. Spec, gels, send to Jeff B. Shipping? <br>
  <br>
  <br>
† COBRA <br>
† COBRA <br>
* Mark- Control circuit? <br>  
* Mark- Control circuit? <br>  
* Bacteria Experiment - Update from Jane and Jaephil? <br>
* Bacteria Experiment - Changing the teflon filter. <br>
* Substrates - JZ to try with more gold and do all SEM/sample testing.  <br>
* Pluronic results? - straight channels. Treated/non, pluronic better. NEED UPDATE FROM JD <br>
* Substrates - JZ tried with more gold and do all SEM/sample testing.  <br>
<br>
<br>
† Valve Array/Valve building (FJ) <br>
† Valve Array/Valve building (FJ) <br>
* Status from Mark? <br>
* Status from Mark? - valves in transit somewhere. <br>
* Frank should talk with Alex, Mark, and Jaephil and make sure we are not duplicating efforts/making something that is too big for portability.<br>
* Frank should talk with Alex, Mark, and Jaephil and make sure we are not duplicating efforts/making something that is too big for portability.<br>
* Frank met with Mark on Friday, has concerns along the lines of the above comment (duplicating efforts etc.) that will hopefully be addressed during/after lab meeting. <br>
<br>
<br>
† Fraunhofer: LOAC <br>
† Fraunhofer: LOAC <br>
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<br>
<br>
† Biointerfaces group <br>
† Biointerfaces group <br>
* Paul Mak told that Mask aligner at Photonics center has UV filter. Try that machine. - UPDATE?<br>
* Mask made. Chrome mask. <br>
* Preliminary E. coli experiment is ongoing. UPDATE? <br>
* Preliminary E. coli experiment is ongoing. <br>
* Try DAPI bacteria stain to visualize e-coli soon. UPDATE? <br>
<br>
<br>
† CIMIT- Colson Grant<br>
† CIMIT- Colson Grant<br>
Line 47: Line 48:
<br>
<br>
† PCR <br>
† PCR <br>
* QQ gel issues. Stop using ABI flu probe that QQ ordered. Try Cathie's old assay before designing a new one. Run gels. <br>
* Cathie look up her flu assay specs. <br>
* Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - Will try after fixes and report next week. UPDATE? <br>
* Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers.<br>
* Look into Melting temp analysis on the 7300 - can only do with Sonali's assay. <br>
* Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit. <br>
'''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. <br>'''
'''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. <br>'''
* MinCheol's desgins.  UPDATE? <br>
* MinCheol's designs. Done. Data looks the same as old design. Extension time not long enough.  <br>
* Will do extension design with lambda phage assay. <br>
* Mincheol will work on DNA migration simulation after IMECE 2008.<br>
* Mincheol will work on DNA migration simulation after IMECE 2008.<br>
<br>
<br>
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<br>
<br>
† Senior project <br>
† Senior project <br>
* Need updates from both Senior project students in bullet form. <br>
* Megan has mask designs, JD will check, send out this week. <br>
* Emailed Teddy. <br>
* Depth of channels, check with JD and Sonali, because Sonali will be able to tell you how much assay volume. <br>
* Mark will help/learn SU8 step. <br>
* Teddy doing off chip controls work. Will consult with Megan and JD to get interface design going. Software control, too..LabVIEW. <br>
* Valves in the mail --- find them! (MARK)<br>
<br>
<br>
† F31: Cochlea <br>
† F31: Cochlea <br>
* Received paper edits from Doug.<br>
* Made changes and gsve to Cathie. <br>
* Make changes and give to Cathie ASAP. <br>
*  
* Journal chosen? <br>
* Need to order Alpha-Tectorin protein.<br>
<br>
<br>
† Silica Optimization (Lambda): <br>
† Silica Optimization (Lambda): <br>
* De-inhibit Methyl Methacrylate (100ml), put in fridge, make new columns. <br>
* Ongoing. More concentrations.  <br>
* Ongoing. More concentrations.  <br>
* Pressure testing on these channels when they are flowing. <br>
* Pico Green assay figured out.<br>
* Ethanol precipitation protocol and preparation of sodium acetate. ASAP. <br>
 


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Revision as of 10:13, 11 November 2008

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11 November 2008 Lab Meeting Agenda

† Hussam's research report today.

† Announcements

  • Your personal priorities have been emailed to you by me. Please take note.


† Flu R01

  • Courier service! Sample transport set up.
  • Plaque assay status -- plaques and everything!Yeah!
  • Mark - status of fixture?


† SEPSIS Project - CIMIT Bird Flu

  • Cathie working on paper.


†RNA project

  • Jeff Braman will visit in early Dec.
  • Do cDNA prep. Spec, gels, send to Jeff B. Shipping?

† COBRA

  • Mark- Control circuit?
  • Bacteria Experiment - Changing the teflon filter.
  • Pluronic results? - straight channels. Treated/non, pluronic better. NEED UPDATE FROM JD
  • Substrates - JZ tried with more gold and do all SEM/sample testing.


† Valve Array/Valve building (FJ)

  • Status from Mark? - valves in transit somewhere.
  • Frank should talk with Alex, Mark, and Jaephil and make sure we are not duplicating efforts/making something that is too big for portability.
  • Frank met with Mark on Friday, has concerns along the lines of the above comment (duplicating efforts etc.) that will hopefully be addressed during/after lab meeting.


† Fraunhofer: LOAC

  • Primer/dimer issue. Talked with QQ. We do not appear to have this problem with our chips.


† Biointerfaces group

  • Mask made. Chrome mask.
  • Preliminary E. coli experiment is ongoing.


† CIMIT- Colson Grant

  • Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
  • STAR-CD Renewal - January ($2K)


† PCR

  • Cathie look up her flu assay specs.
  • Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers.
  • Look into Melting temp analysis on the 7300 - can only do with Sonali's assay.
  • Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit.

* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture.

  • MinCheol's designs. Done. Data looks the same as old design. Extension time not long enough.
  • Will do extension design with lambda phage assay.
  • Mincheol will work on DNA migration simulation after IMECE 2008.


† RCA

  • Cathie submitted One pager Whitepaper. Waiting for response.


† Senior project

  • Megan has mask designs, JD will check, send out this week.
  • Depth of channels, check with JD and Sonali, because Sonali will be able to tell you how much assay volume.
  • Mark will help/learn SU8 step.
  • Teddy doing off chip controls work. Will consult with Megan and JD to get interface design going. Software control, too..LabVIEW.
  • Valves in the mail --- find them! (MARK)


† F31: Cochlea

  • Made changes and gsve to Cathie.


† Silica Optimization (Lambda):

  • De-inhibit Methyl Methacrylate (100ml), put in fridge, make new columns.
  • Ongoing. More concentrations.
  • Pico Green assay figured out.
  • Ethanol precipitation protocol and preparation of sodium acetate. ASAP.



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