11 November 2008 Lab Meeting Agenda
† Hussam's research report today.
† Announcements
- Your personal priorities have been emailed to you by me. Please take note.
† Flu R01
- Courier service! Sample transport set up.
- Plaque assay status?
- Mark - status of fixture?
† SEPSIS Project - CIMIT Bird Flu
†RNA project
- Jeff Braman will visit in early Dec.
- Do cDNA prep. Spec, gels, send to Jeff B.
† COBRA
- Mark- Control circuit?
- Bacteria Experiment - Update from Jane and Jaephil?
- Substrates - JZ to try with more gold and do all SEM/sample testing.
† Valve Array/Valve building (FJ)
- Status from Mark?
- Frank should talk with Alex, Mark, and Jaephil and make sure we are not duplicating efforts/making something that is too big for portability.
- Frank met with Mark on Friday, has concerns along the lines of the above comment (duplicating efforts etc.) that will hopefully be addressed during/after lab meeting.
† Fraunhofer: LOAC
- Primer/dimer issue. Talked with QQ. We do not appear to have this problem with our chips.
† Biointerfaces group
- Paul Mak told that Mask aligner at Photonics center has UV filter. Try that machine. - UPDATE?
- Preliminary E. coli experiment is ongoing. UPDATE?
- Try DAPI bacteria stain to visualize e-coli soon. UPDATE?
† CIMIT- Colson Grant
- Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
- STAR-CD Renewal - January ($2K)
† PCR
- cathie look up her flu assay specs.
- Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers.
- Look into Melting temp analysis on the 7300 - can only do with Sonali's assay.
- QQ gel issues. Stop using ABI flu probe that QQ ordered. Try Cathie's old assay before designing a new one. Run gels.
- Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - Will try after fixes and report next week. UPDATE?
* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture.
- MinCheol's designs. UPDATE?
- Mincheol will work on DNA migration simulation after IMECE 2008.
† RCA
- Cathie submitted One pager Whitepaper. Waiting for response.
† Senior project
- Megan has mask designs, JD will check, send out this week.
- Depth of channels, check with JD and Sonali, because Sonali will be able to tell you how much assay volume.
- Mark will help/learn SU8 step.
- Teddy doing off chip controls work. Will consult with Megan and JD to get interface design going. Software control, too..LabVIEW.
- Valves in the mail --- find them! (MARK)
† F31: Cochlea
- Made changes and gsve to Cathie.
† Silica Optimization (Lambda):
- De-inhibit Methyl Methacrylate (100ml), put in fridge, make new columns.
- Ongoing. More concentrations.
- Pico Green assay figured out.
- Ethanol precipitation protocol and preparation of sodium acetate. ASAP.
|