Klapperich Lab:Notebook/Lab Meeting Notes/2008/11/18: Difference between revisions

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* Plaque Assay? <br>
* Plaque Assay? <br>
* Mark - status of fixture? <br>
* Mark - status of fixture? <br>
* hussam: Flu fixture will be ready 11-19-08 (tomorrow) <br>
<br>
<br>
† SEPSIS Project - CIMIT Bird Flu <br>
† SEPSIS Project - CIMIT Bird Flu <br>
Line 72: Line 74:
<br>
<br>
† Silica Optimization (Lambda): <br>
† Silica Optimization (Lambda): <br>
* UPDATE? <br>
* UPDATE 11-18-08 <br>
* De-inhibit Methyl Methacrylate (100ml), put in fridge, make new columns. <br>
* De-inhibit Methyl Methacrylate (100ml), put in fridge, make new columns. (today) <br>
* Ongoing. More concentrations. <br>
* Ongoing. More concentrations. saved samples in -20 until pellet paint figured out <br>
* Pico Green assay figured out.<br>
* Repeating experiments this week with activated MMA and collecting more samples <br>
* Ethanol precipitation protocol and preparation of sodium acetate. ASAP. <br>
* Plates used for Pico Green are not sterile ?? (problem? yes? no?) <br>
* Pico Green assay figured out and standards working good.<br>
* Pellet paint: Even (-) ctrl form a pellet , after 100% ethanol wash pellet is detached.<br>


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Revision as of 10:06, 18 November 2008

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18 November 2008 Lab Meeting Agenda

† Alex's research report today.

† Announcements

  • Room 720 move out.


† Flu R01

  • First samples taken starting tomorrow, 11/19.
  • Plaque Assay?
  • Mark - status of fixture?
  • hussam: Flu fixture will be ready 11-19-08 (tomorrow)


† SEPSIS Project - CIMIT Bird Flu

  • Cathie STILL working on paper.


†RNA project

  • Jeff Braman will visit December 12th
  • Do cDNA prep. Spec, gels, send to Jeff B. Shipping?

† COBRA

  • Mark- Control circuit?
  • Bacteria Experiment - Changing channel size?
  • Substrates ?


† Valve Array/Valve building (FJ)

  • Status from Mark? - valves in transit somewhere.



† Fraunhofer: LOAC




† Biointerfaces group

  • Mask made. Chrome mask.
  • E. Coli experiments with fluorescence?


† CIMIT- Colson Grant

  • Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
  • STAR-CD Renewal - January ($2K)


† PCR

  • Cathie's flu assay has nonspecific products, too.
  • Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers. UPDATE?
  • Look into Melting temp analysis on the 7300 - can only do with Sonali's assay.
  • Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit.

* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture.

  • Designed new lambda phage assay with longer product.
  • What do we need to finish the paper?
  • Mincheol will work on DNA migration simulation after IMECE 2008.


† RCA

  • Cathie submitted One pager Whitepaper. Waiting for response.


† Senior project

  • Megan has mask designs, JD will check, send out this week.
  • Depth of channels, check with JD and Sonali, because Sonali will be able to tell you how much assay volume.
  • Mark will help/learn SU8 step.
  • Teddy doing off chip controls work. Will consult with Megan and JD to get interface design going. Software control, too..LabVIEW.
  • Valves in the mail --- find them! (MARK)


† F31: Cochlea

  • Submit paper.


† Silica Optimization (Lambda):

  • UPDATE 11-18-08
  • De-inhibit Methyl Methacrylate (100ml), put in fridge, make new columns. (today)
  • Ongoing. More concentrations. saved samples in -20 until pellet paint figured out
  • Repeating experiments this week with activated MMA and collecting more samples
  • Plates used for Pico Green are not sterile ?? (problem? yes? no?)
  • Pico Green assay figured out and standards working good.
  • Pellet paint: Even (-) ctrl form a pellet , after 100% ethanol wash pellet is detached.


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