Klapperich Lab:Notebook/Lab Meeting Notes/2008/11/18: Difference between revisions

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==18 November 2008 Lab Meeting Agenda==
==18 November 2008 Lab Meeting Agenda==
† Hussam's research report today.
† Alex's research report today.


† Announcements<br>
† Announcements<br>
* Your personal priorities have been emailed to you by me. Please take note.<br>
* Room 720 move out. <br>
<br>
<br>
† Flu R01 <br>
† Flu R01 <br>
* Courier service! Sample transport set up. <br>
* First samples taken starting tomorrow, 11/19. <br>
* Plaque assay status -- plaques and everything!Yeah! <br>
* Plaque Assay? <br>
* Mark - status of fixture? <br>
* Mark - status of fixture? <br>
<br>
<br>
† SEPSIS Project - CIMIT Bird Flu <br>
† SEPSIS Project - CIMIT Bird Flu <br>
* Cathie working on paper.<br>
* Cathie STILL working on paper.<br>
<br>
<br>
†RNA project
†RNA project
* Jeff Braman will visit in early Dec. <br>
* Jeff Braman will visit December 12th <br>
* Do cDNA prep. Spec, gels, send to Jeff B. Shipping? <br>
* Do cDNA prep. Spec, gels, send to Jeff B. Shipping? <br>
  <br>
  <br>
† COBRA <br>
† COBRA <br>
* Mark- Control circuit? <br>  
* Mark- Control circuit? <br>  
* Bacteria Experiment - Changing the teflon filter. <br>
* Bacteria Experiment - Changing channel size? <br>
* Pluronic results? - straight channels. Treated/non, pluronic better. NEED UPDATE FROM JD <br>
* Substrates ? <br>
* Substrates - JZ tried with more gold and do all SEM/sample testing.  <br>
<br>
<br>
† Valve Array/Valve building (FJ) <br>
† Valve Array/Valve building (FJ) <br>
* Status from Mark? - valves in transit somewhere. <br>
* Status from Mark? - valves in transit somewhere. <br>
* Frank should talk with Alex, Mark, and Jaephil and make sure we are not duplicating efforts/making something that is too big for portability.<br>
* <br>
* Frank met with Mark on Friday, has concerns along the lines of the above comment (duplicating efforts etc.) that will hopefully be addressed during/after lab meeting. <br>
* <br>
<br>
<br>
† Fraunhofer: LOAC <br>
† Fraunhofer: LOAC <br>
* Primer/dimer issue. Talked with QQ. We do not appear to have this problem with our chips. <br>
* <br>
*  <br>
*  <br>
<br>
<br>
† Biointerfaces group <br>
† Biointerfaces group <br>
* Mask  made. Chrome mask. <br>
* Mask  made. Chrome mask. <br>
* Preliminary E. coli experiment is ongoing. <br>
* E. Coli experiments with fluorescence? <br>
<br>
<br>
† CIMIT- Colson Grant<br>
† CIMIT- Colson Grant<br>
Line 49: Line 48:
<br>
<br>
† PCR <br>
† PCR <br>
* Cathie look up her flu assay specs. <br>
* Cathie's flu assay has nonspecific products, too. <br>
* Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers.<br>
* Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers. UPDATE?<br>
* Look into Melting temp analysis on the 7300 - can only do with Sonali's assay. <br>
* Look into Melting temp analysis on the 7300 - can only do with Sonali's assay. <br>
* Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit. <br>
* Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit. <br>
'''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. <br>'''
'''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. <br>'''
* MinCheol's designs. Done. Data looks the same as old design. Extension time not long enough.  <br>
* Designed new lambda phage assay with longer product.  <br>
* Will do extension design with lambda phage assay. <br>
* What do we need to finish the paper? <br>
* Mincheol will work on DNA migration simulation after IMECE 2008.<br>
* Mincheol will work on DNA migration simulation after IMECE 2008.<br>
<br>
<br>
Line 69: Line 68:
<br>
<br>
† F31: Cochlea <br>
† F31: Cochlea <br>
* Made changes and gsve to Cathie. <br>
* Submit paper. <br>
*  
*  
<br>
<br>
† Silica Optimization (Lambda): <br>
† Silica Optimization (Lambda): <br>
* UPDATE? <br>
* De-inhibit Methyl Methacrylate (100ml), put in fridge, make new columns. <br>
* De-inhibit Methyl Methacrylate (100ml), put in fridge, make new columns. <br>
* Ongoing. More concentrations.  <br>
* Ongoing. More concentrations.  <br>

Revision as of 09:14, 18 November 2008

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18 November 2008 Lab Meeting Agenda

† Alex's research report today.

† Announcements

  • Room 720 move out.


† Flu R01

  • First samples taken starting tomorrow, 11/19.
  • Plaque Assay?
  • Mark - status of fixture?


† SEPSIS Project - CIMIT Bird Flu

  • Cathie STILL working on paper.


†RNA project

  • Jeff Braman will visit December 12th
  • Do cDNA prep. Spec, gels, send to Jeff B. Shipping?

† COBRA

  • Mark- Control circuit?
  • Bacteria Experiment - Changing channel size?
  • Substrates ?


† Valve Array/Valve building (FJ)

  • Status from Mark? - valves in transit somewhere.



† Fraunhofer: LOAC




† Biointerfaces group

  • Mask made. Chrome mask.
  • E. Coli experiments with fluorescence?


† CIMIT- Colson Grant

  • Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
  • STAR-CD Renewal - January ($2K)


† PCR

  • Cathie's flu assay has nonspecific products, too.
  • Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers. UPDATE?
  • Look into Melting temp analysis on the 7300 - can only do with Sonali's assay.
  • Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit.

* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture.

  • Designed new lambda phage assay with longer product.
  • What do we need to finish the paper?
  • Mincheol will work on DNA migration simulation after IMECE 2008.


† RCA

  • Cathie submitted One pager Whitepaper. Waiting for response.


† Senior project

  • Megan has mask designs, JD will check, send out this week.
  • Depth of channels, check with JD and Sonali, because Sonali will be able to tell you how much assay volume.
  • Mark will help/learn SU8 step.
  • Teddy doing off chip controls work. Will consult with Megan and JD to get interface design going. Software control, too..LabVIEW.
  • Valves in the mail --- find them! (MARK)


† F31: Cochlea

  • Submit paper.


† Silica Optimization (Lambda):

  • UPDATE?
  • De-inhibit Methyl Methacrylate (100ml), put in fridge, make new columns.
  • Ongoing. More concentrations.
  • Pico Green assay figured out.
  • Ethanol precipitation protocol and preparation of sodium acetate. ASAP.


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