Klapperich Lab:Notebook/Lab Meeting Notes/2008/12/09: Difference between revisions

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==9 December 2008 Lab Meeting Agenda==
† Stephanie's research report today.


† Announcements<br>
* Meeting on 12/16.
* No meeting on 12/23 <br>
<br>
† Flu R01 <br>
* First samples taken starting next week, 11/19. <br>
* Sample training 9:30am Wed. <br>
* Plaque Assay - first samples today. Ref stock 2x10^4 pfu/ml <br>
* Mark trained for real to make SU molds. Trained to make the monoliths. <br>
* Hussam: Flu fixture will be ready 11-19-08 (tomorrow)- 6 channel fixture (2). <br>
*
<br>
† SEPSIS Project - CIMIT Bird Flu <br>
* Cathie STILL working on paper.<br>
<br>
†RNA project
* Jeff Braman will visit December 12th <br>
* Do cDNA prep. Spec, gels, send to Jeff B. Shipping? <br>
<br>
† COBRA <br>
* Mark- Control circuit? <br>
* Bacteria Experiment - Changing channel size. <br>
* Substrates -SEM down. <br>
<br>
† Valve Array/Valve building (FJ) <br>
* Valves backorderd until 24th. <br>
* <br>
* <br>
<br>
† Fraunhofer: LOAC <br>
* <br>
<br>
† Biointerfaces group <br>
* Mask  made. Chrome mask. <br>
* E. Coli experiments with fluorescence? <br>
* Aim for fluor data for 6th. <br>
<br>
† CIMIT- Colson Grant<br>
* Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09<br>
* STAR-CD Renewal - January ($2K)<br>
<br>
† PCR <br>
* Cathie's flu assay has nonspecific products, too. <br>
* Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers. UPDATE?<br>
* Look into Melting temp analysis on the 7300  - Done. what is contaminated? <br>
* Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit. <br>
'''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. <br>'''
* Design new lambda phage assay with longer product.  <br>
* What do we need to finish the paper? <br>
* <br>
<br>
† RCA <br>
* Cathie submitted One pager Whitepaper. Waiting for response. <br>
<br>
† Senior project <br>
* Megan has mask designs. CHECK WITH HER. <br>
* Depth of channels, check with JD and Sonali, because Sonali will be able to tell you how much assay volume. 100 microns. <br>
* Mark will help/learn SU8 step. <br>
* Teddy doing off chip controls work.  <br>
<br>
<br>
† F31: Cochlea <br>
* Submit paper. <br>
*
<br>
† Silica Optimization (Lambda): <br>
* UPDATE 11-18-08 <br>
* De-inhibit Methyl Methacrylate (100ml), put in fridge, make new columns. (today) <br>
* Ongoing. More concentrations. saved samples in -20 until pellet paint figured out <br>
* Repeating experiments this week with activated MMA and collecting more samples <br>
* Plates used for Pico Green are not sterile ?? NOT LIKELY. <br>
* Pico Green assay figured out and standards working.<br>
* Pellet paint: Even (-) ctrl form a pellet , after 100% ethanol wash pellet is detached.<br>
* try std. etoh protocol. <br>
*


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9 December 2008 Lab Meeting Agenda

† Stephanie's research report today.

† Announcements

  • Meeting on 12/16.
  • No meeting on 12/23


† Flu R01

  • First samples taken starting next week, 11/19.
  • Sample training 9:30am Wed.
  • Plaque Assay - first samples today. Ref stock 2x10^4 pfu/ml
  • Mark trained for real to make SU molds. Trained to make the monoliths.
  • Hussam: Flu fixture will be ready 11-19-08 (tomorrow)- 6 channel fixture (2).


† SEPSIS Project - CIMIT Bird Flu

  • Cathie STILL working on paper.


†RNA project

  • Jeff Braman will visit December 12th
  • Do cDNA prep. Spec, gels, send to Jeff B. Shipping?

† COBRA

  • Mark- Control circuit?
  • Bacteria Experiment - Changing channel size.
  • Substrates -SEM down.


† Valve Array/Valve building (FJ)

  • Valves backorderd until 24th.



† Fraunhofer: LOAC



† Biointerfaces group

  • Mask made. Chrome mask.
  • E. Coli experiments with fluorescence?
  • Aim for fluor data for 6th.


† CIMIT- Colson Grant

  • Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
  • STAR-CD Renewal - January ($2K)


† PCR

  • Cathie's flu assay has nonspecific products, too.
  • Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers. UPDATE?
  • Look into Melting temp analysis on the 7300 - Done. what is contaminated?
  • Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit.

* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture.

  • Design new lambda phage assay with longer product.
  • What do we need to finish the paper?


† RCA

  • Cathie submitted One pager Whitepaper. Waiting for response.


† Senior project

  • Megan has mask designs. CHECK WITH HER.
  • Depth of channels, check with JD and Sonali, because Sonali will be able to tell you how much assay volume. 100 microns.
  • Mark will help/learn SU8 step.
  • Teddy doing off chip controls work.



† F31: Cochlea

  • Submit paper.


† Silica Optimization (Lambda):

  • UPDATE 11-18-08
  • De-inhibit Methyl Methacrylate (100ml), put in fridge, make new columns. (today)
  • Ongoing. More concentrations. saved samples in -20 until pellet paint figured out
  • Repeating experiments this week with activated MMA and collecting more samples
  • Plates used for Pico Green are not sterile ?? NOT LIKELY.
  • Pico Green assay figured out and standards working.
  • Pellet paint: Even (-) ctrl form a pellet , after 100% ethanol wash pellet is detached.
  • try std. etoh protocol.


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