Klapperich Lab:Notebook/Lab Meeting Notes/2008/12/16

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(16 December 2008 Lab Meeting Agenda)
(16 December 2008 Lab Meeting Agenda)
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* Mark- Control circuit  - This will be done by January.<br>  
* Mark- Control circuit  - This will be done by January.<br>  
* Evap quantification experiments ongoing. Still working on live/dead assay. (JD, JZ)<br>
* Evap quantification experiments ongoing. Still working on live/dead assay. (JD, JZ)<br>
 +
- problems: dead but green in the end <br>
 +
- called tech support. <br>
 +
- tests: supernatant fluorescent? excessive dye to observe time-lapse change in green/red ratio. <br>
 +
- omit washing step before dilution: wash, dye, incubate, dilute, instead of wash, dye, incubate, wash, dilute <br>
 +
* Substrates (JZ)<br>
* Substrates (JZ)<br>
 +
- new set made, in glove box: the 1st 3 on the DOE. SERS signal strong, SEM today <br>
* DOE for the experiment. <br>
* DOE for the experiment. <br>
 +
- need to add humidity as it changes even in the glove box <br>
* Colloid experiment. inconclusive. Look at SEMs <br>
* Colloid experiment. inconclusive. Look at SEMs <br>
 +
- difficulty in locating the bacteria <br>
 +
- needs much higher particle/bacteria ratio <br>
 +
- experiments outside the channel first? <br>
 +
* Prospectus framework/discussion: target the end of this week <br>
<br>
<br>
 +
† Valve Array/Valve building (FJ) <br>
† Valve Array/Valve building (FJ) <br>
* Turn over platform to Teddy <br>
* Turn over platform to Teddy <br>

Revision as of 11:01, 16 December 2008

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16 December 2008 Lab Meeting Agenda

  • check with b&G about compressed air and vacuum?

† Announcements

  • Put travel dates on lab calendar ASAP.
    .
  • No meeting on 12/30
  • Meeting Time for Spring Semester (starting week of 1/16). Put your NOT OPEN times here.

Cathie T/TH 12-2pm.
Jane T 3-5
Nathan F 10-12
Megan Mon (9am-6pm), Tues (1-4pm), Wed (9am-2pm)
Frank has OPEN M 12-4PM, T/TH before 10AM, W-F after 3PM, F 11AM-1PM
Hussam Mon, (10-2 pm), (Wed 10-5), (Fri 10-12) ,(Tue Thur 10-12 pm)
Sonali MWF 11am -1pm. Mincheol Mon-Fri 9am-1pm
† Flu R01

  • First samples ?
  • Plaque Assay
  • Alex needs to be trained to make SU molds. Trained to make the monoliths (by Hussam By 1/15/09).
  • Hussam: Flu fixture done.


† SEPSIS Project

  • Sonali working on first draft of new version paper by 12/25. Journal?


†RNA project

  • Jeff Braman will visit December 12th. 9:30am Visit to Cathie's lab and lab tour. Meetings starting at 10am in Fraunhofer. Sonali and Cathie to attend.
  • Do cDNA prep. Spec, gels, send to Jeff B. Shipping?

† COBRA

  • Mark- Control circuit - This will be done by January.
  • Evap quantification experiments ongoing. Still working on live/dead assay. (JD, JZ)

- problems: dead but green in the end
- called tech support.
- tests: supernatant fluorescent? excessive dye to observe time-lapse change in green/red ratio.
- omit washing step before dilution: wash, dye, incubate, dilute, instead of wash, dye, incubate, wash, dilute

  • Substrates (JZ)

- new set made, in glove box: the 1st 3 on the DOE. SERS signal strong, SEM today

  • DOE for the experiment.

- need to add humidity as it changes even in the glove box

  • Colloid experiment. inconclusive. Look at SEMs

- difficulty in locating the bacteria
- needs much higher particle/bacteria ratio
- experiments outside the channel first?

  • Prospectus framework/discussion: target the end of this week


† Valve Array/Valve building (FJ)

  • Turn over platform to Teddy



† Fraunhofer: LOAC

  • So close!


† Biointerfaces group


  • E. Coli experiments with fluorescence.
  • Strobe experiments? Jason cannot do them, perhaps MCK can. MCK will talk to Jason this week.
  • Made new design for trapper.
  • Mask writer was broken.


† CIMIT- Colson Grant

  • First pages of first IRB started last week. Target date 1/1/09
  • STAR-CD Renewal - January ($2K)


† PCR


  • Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers. UPDATE?
  • Look into Melting temp analysis on the 7300 - Done. what is contaminated?
  • Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit. ON HOLD

* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. ON HOLD

  • Try microscope for looking a fluor.
  • 52, 60, 65 C repeats.


† RCA

  • Cathie submitted One pager Whitepaper. Waiting for response.


† Senior project

  • Megan 1)did some preliminary bonding with Zeonex and teflon film. 2) emailed off the Mask Design to FineLine

† F31: Cochlea

  • Paper submitted.
  • set up adviser meeting Jan


† Silica Optimization (Lambda):

  • inhibition infor to Paul.
  • Replot the data so I can read it. ASAP
  • Do three elutions.
  • Next E. coli.


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