Klapperich Lab:Notebook/Lab Meeting Notes/2009/01/08

8 January 2009 Lab Meeting Agenda

• Check with B&G about compressed air and vacuum?

† Announcements

• Meeting Time for Spring Semester is THURSDAYS 3-5pm ROOM 705. Stating 1/8/09. See Lab Calendar for dates and presentation schedule.

† Flu R01

• Plaque Assay.
  
• Alex needs to be trained to make SU molds. Trained to make the monoliths (by Hussam By 1/15/09).
  

† SEPSIS Project

• Sonali working on first draft of new version paper by 12/25. Journal?
  
• Hussam working on Lambda phage data.
  

†RNA project

• More formal report to Jeff from Cathie.
  
• Do cDNA prep. Spec, gels, send to Jeff B. Shipping?
  

† COBRA

• Mark- Control circuit - This will be done by January.
  
• Evap quantification experiments ongoing. Still working on live/dead assay.(JD, JZ)
  

- problems: dead but green in the end
- called tech support.
- tests: supernatant fluorescent? excessive dye to observe time-lapse change in green/red ratio.
- omit washing step before dilution: wash, dye, incubate, dilute, instead of wash, dye, incubate, wash, dilute
- try running the experiment first, then running the live/dead dye through second.
- Petridish culture after running evaporation w/o dye in hydrophobic treated channel.

• Hydrophobic coating of microchannel

- RIE (CHF3), Rain?, and Cytop coated and thermal bonding with PTFE filter tested.
- Cytop turned out good bonding with PTFE filter.

• Test cheap wait for running
  
• Slide for dispensing or integration scheme with SERS substrate
  

• Substrates (JZ)
  

- new set made, in glove box: the 1st 3 on the DOE. SEM and SERS available.
- hypothesis: drying controls clustering, reduction controls size?

• DOE for the experiment.
  

- need to add humidity as it changes even in the glove box - this is no longer an issue, asked Ranjith and now can adjust humidity to constant
- set II done varying drying time and fast reduction time. SEM and SERS available.

• Colloid experiment. inconclusive. Look at SEMs
  

- difficulty in locating the bacteria
- needs much higher particle/bacteria ratio
- experiments outside the channel first?

• Prospectus framework/discussion: target the end of this week 1/23/09.
  

† Valve Array/Valve building (FJ)

• Turn over platform to Teddy
  
• TEDDY needs to meet with Mark.
  

† Fraunhofer: LOAC

• So so close!
  

† Biointerfaces group

• 
  
• Strobe experiments? Jason cannot do them, perhaps MCK can. MCK will talk to Jason this week.
  
• Made new design for trapper.
  
• Mask writer was broken.
  

† CIMIT- Colson Grant

• IRB still going/ revisions by tomorrow to Ian. Target date 1/1/09
  
• STAR-CD Renewal - January ($2K)
  

† PCR

• Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit. ON HOLD
  

* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. ON HOLD

• Try microscope for looking a fluor. Learn how to look at the data in Image J
  
• 
  

† RCA

• Cathie submitted One pager Whitepaper. Waiting for response.
  

† Senior project

• Megan 1)did some preliminary bonding with Zeonex and teflon film. 2) emailed off the Mask Design to FineLine
  
• 
  

† F31: Cochlea

• Set up adviser meeting Jan
  
• Post Gent vs. Pre gent - 6 proteins. 4/6 no change.
  
• Final two proteins: know by week of 1/19

† Silica Optimization (Lambda):

• Update by 12/24.
  
• Do three elutions.
  
• Next E. coli.