Klapperich Lab:Notebook/Lab Meeting Notes/2009/01/08

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(8 January 2009 Lab Meeting Agenda)
(8 January 2009 Lab Meeting Agenda)
Line 44: Line 44:
* Substrates (JZ)<br>
* Substrates (JZ)<br>
-
- new set made, in glove box: the 1st 3 on the DOE. SERS signal strong, SEM today <br>
+
- new set made, in glove box: the 1st 3 on the DOE. SEM and SERS available. <br>
 +
- hypothesis: drying controls clustering, reduction controls size? <br>
* DOE for the experiment. <br>
* DOE for the experiment. <br>
-
- need to add humidity as it changes even in the glove box <br>
+
- need to add humidity as it changes even in the glove box - this is no longer an issue, asked Ranjith and now can adjust humidity to constant <br>
 +
- set II done varying drying time and fast reduction time. SEM and SERS available. <br>
* Colloid experiment. inconclusive. Look at SEMs <br>
* Colloid experiment. inconclusive. Look at SEMs <br>
- difficulty in locating the bacteria <br>
- difficulty in locating the bacteria <br>

Revision as of 14:15, 8 January 2009

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8 January 2009 Lab Meeting Agenda

  • Check with B&G about compressed air and vacuum?

† Announcements

  • Meeting Time for Spring Semester is THURSDAYS 3-5pm ROOM 705. Stating 1/8/09. See Lab Calendar for dates and presentation schedule.


† Flu R01

  • Plaque Assay.
  • Alex needs to be trained to make SU molds. Trained to make the monoliths (by Hussam By 1/15/09).


† SEPSIS Project

  • Sonali working on first draft of new version paper by 12/25. Journal?
  • Hussam working on Lambda phage data.


†RNA project

  • More formal report to Jeff from Cathie.
  • Do cDNA prep. Spec, gels, send to Jeff B. Shipping?

† COBRA

  • Mark- Control circuit - This will be done by January.
  • Evap quantification experiments ongoing. Still working on live/dead assay.(JD, JZ)

- problems: dead but green in the end
- called tech support.
- tests: supernatant fluorescent? excessive dye to observe time-lapse change in green/red ratio.
- omit washing step before dilution: wash, dye, incubate, dilute, instead of wash, dye, incubate, wash, dilute
- try running the experiment first, then running the live/dead dye through second.
- Petridish culture after running evaporation w/o dye in hydrophobic treated channel.

  • Hydrophobic coating of microchannel

- RIE (CHF3), Rain?, and Cytop coated and thermal bonding with PTFE filter tested.
- Cytop turned out good bonding with PTFE filter.

  • Test cheap wait for running
  • Slide for dispensing or integration scheme with SERS substrate


  • Substrates (JZ)

- new set made, in glove box: the 1st 3 on the DOE. SEM and SERS available.
- hypothesis: drying controls clustering, reduction controls size?

  • DOE for the experiment.

- need to add humidity as it changes even in the glove box - this is no longer an issue, asked Ranjith and now can adjust humidity to constant
- set II done varying drying time and fast reduction time. SEM and SERS available.

  • Colloid experiment. inconclusive. Look at SEMs

- difficulty in locating the bacteria
- needs much higher particle/bacteria ratio
- experiments outside the channel first?

  • Prospectus framework/discussion: target the end of this week 1/23/09.


† Valve Array/Valve building (FJ)

  • Turn over platform to Teddy
  • TEDDY needs to meet with Mark.


† Fraunhofer: LOAC

  • So so close!


† Biointerfaces group


  • Strobe experiments? Jason cannot do them, perhaps MCK can. MCK will talk to Jason this week.
  • Made new design for trapper.
  • Mask writer was broken.


† CIMIT- Colson Grant

  • IRB still going/ revisions by tomorrow to Ian. Target date 1/1/09
  • STAR-CD Renewal - January ($2K)


† PCR

  • Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit. ON HOLD

* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. ON HOLD

  • Try microscope for looking a fluor. Learn how to look at the data in Image J


† RCA

  • Cathie submitted One pager Whitepaper. Waiting for response.


† Senior project

  • Megan 1)did some preliminary bonding with Zeonex and teflon film. 2) emailed off the Mask Design to FineLine

† F31: Cochlea

  • Set up adviser meeting Jan
  • Post Gent vs. Pre gent - 6 proteins. 4/6 no change.
  • Final two proteins: know by week of 1/19


† Silica Optimization (Lambda):

  • Update by 12/24.
  • Do three elutions.
  • Next E. coli.


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