Klapperich Lab:Notebook/Lab Meeting Notes/2009/01/08

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(8 January 2009 Lab Meeting Agenda)
(8 January 2009 Lab Meeting Agenda)
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* <br>
* <br>
* Strobe experiments? Jason cannot do them, perhaps MCK can. MCK will talk to Jason this week. <br>
* Strobe experiments? Jason cannot do them, perhaps MCK can. MCK will talk to Jason this week. <br>
* Made new design for trapper. <br>
* CR mask was printed new design for trapper . <br>
* Mask writer was broken. <br>
* UV filter installed <br>
† CIMIT- Colson Grant<br>
† CIMIT- Colson Grant<br>

Revision as of 15:28, 8 January 2009

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8 January 2009 Lab Meeting Agenda

  • Check with B&G about compressed air and vacuum?

† Announcements

  • Meeting Time for Spring Semester is THURSDAYS 3-5pm ROOM 705. Stating 1/8/09. See Lab Calendar for dates and presentation schedule.

† Flu R01

  • Plaque Assay.
  • Alex needs to be trained to make SU molds. Trained to make the monoliths (by Hussam By 1/15/09)--we're about to start the training--.

† SEPSIS Project

  • Sonali working on first draft of new version paper by 12/25. Journal?
  • Hussam working on Lambda phage data.

†RNA project

  • More formal report to Jeff from Cathie.
  • Do cDNA prep. Spec, gels, send to Jeff B. Shipping?


  • Mark- Control circuit - This will be done by January.
  • Evap quantification experiments ongoing. Still working on live/dead assay.(JD, JZ)

- problems: dead but green in the end
- called tech support.
- tests: supernatant fluorescent? excessive dye to observe time-lapse change in green/red ratio.
- omit washing step before dilution: wash, dye, incubate, dilute, instead of wash, dye, incubate, wash, dilute
- try running the experiment first, then running the live/dead dye through second.
- Petridish culture after running evaporation w/o dye in hydrophobic treated channel.

  • Hydrophobic coating of microchannel

- RIE (CHF3), Rain?, and Cytop coated and thermal bonding with PTFE filter tested.
- Cytop turned out good bonding with PTFE filter.

  • Test cheap wait for running
  • Slide for dispensing or integration scheme with SERS substrate

  • Substrates (JZ)

- new set made, in glove box: the 1st 3 on the DOE. SEM and SERS available.
- hypothesis: drying controls clustering, reduction controls size?

  • DOE for the experiment.

- need to add humidity as it changes even in the glove box - this is no longer an issue, asked Ranjith and now can adjust humidity to constant
- set II done varying drying time and fast reduction time. SEM and SERS available.

  • Colloid experiment. inconclusive. Look at SEMs

- difficulty in locating the bacteria
- needs much higher particle/bacteria ratio
- experiments outside the channel first?

  • Prospectus framework/discussion: target the end of this week 1/23/09.

† Valve Array/Valve building (FJ)

  • Turn over platform to Teddy
  • TEDDY needs to meet with Mark.

† Fraunhofer: LOAC

  • So so close!

† Biointerfaces group

  • Strobe experiments? Jason cannot do them, perhaps MCK can. MCK will talk to Jason this week.
  • CR mask was printed new design for trapper .
  • UV filter installed

† CIMIT- Colson Grant

  • IRB still going/ revisions by tomorrow to Ian. Target date 1/1/09
  • STAR-CD Renewal - January ($2K)


  • Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit. ON HOLD

* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. ON HOLD

  • Try microscope for looking a fluor. Learn how to look at the data in Image J


  • Cathie submitted One pager Whitepaper. Waiting for response.

† Senior project

  • Megan 1)did some preliminary bonding with Zeonex and teflon film. 2) emailed off the Mask Design to FineLine

† F31: Cochlea

  • Set up adviser meeting Jan
  • Post Gent vs. Pre gent - 6 proteins. 4/6 no change.
  • Final two proteins: know by week of 1/19

† Silica Optimization (Lambda):

  • Elution of C and D total DNA mass 2ng and 0.2 ng [1]
  • Testing with water 12 channels- standard protocol- NA replaced with NF water [2]
  • 10 channels - 5ch methanol-water / 5ch methanol-buffer-water [3]
  • Next E. coli.

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