Klapperich Lab:Notebook/Lab Meeting Notes/2009/02/12: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2009/02/12 Entry for Klapperich_Lab:Notebook/Lab_Meeting_Notes)
 
No edit summary
(11 intermediate revisions by 6 users not shown)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==12 February 2009 Lab Meeting Agenda==


* Status: compressed air and vacuum? Why did the work not happen?


† Announcements<br>
* Sonali out for a week.<br>
* New senior project team. <br>
*MicroTAS abstracts. <br>
<br>
† Flu R01 <br>
* Modified Schedule this week. <br>
* Plaque Assay. <br>
* <br>
<br>
† SEPSIS Project <br>
* Sonali done with draft. <br>
*  <br>.
*  <br>
<br>
†RNA project
* More formal report to Jeff from Cathie. <br>
* Do cDNA prep. Spec, gels, send to Jeff B. Shipping? <br>
<br>
<br>
† COBRA <br>
* Mark- Control circuit - done, need to be tested.<br>
* Integration test from here forward br>
- Direct measurement with engineered SERS substrate.<br>
* Test with engineered SERS substrates <br>
- 500umx500umx50um dimension informed to Luca and Bjoern <br>
- Need to schedule when they are ready.<br>


* Substrates (JZ)<br>
- Try glass fixture. <br>
- <br>
- <br>
* Colloid experiment. inconclusive. Look at SEMs <br>
- difficulty in locating the bacteria <br>
- needs much higher particle/bacteria ratio <br>
*<br>
<br>
† Valve Array/Valve building (FJ) <br>
* Teddy on track. <br>
* Valve tested <br>
<br>
† Fraunhofer: LOAC <br>
* Paper drafting!<br>
<br>
† Biointerfaces group <br>
* <br>
* Final experiments in process? Need one more experiment next week <br>
<br>
† CIMIT- Colson Grant<br>
* IRB still in revision.<br>
*<br>
<br>
† PCR <br>
* flu cDNA　ｗａｓ　ｓｕｃｃｅｓｓｆｕｌｌｙ　ａｍｐｌｉｆｉｅｄ　ｏｎ　ｃｈｉｐ，ｏｎｅ　ｂａｎｄ　ｓｈｏｗ　ｕｐ　ｉｎ　ｇｅｌ．　ｅｘｐｅｒｉｍｅｎｔ　ｈａｓ　ｂｅｅｎ　ｒｅｐｅａｔｅｄ　ｆｏｒ　ｔｈｒｅｅ　ｔｉｍｅ．<br>
* ｗｏｒｋｉｎｇ　ｏｎ　ｔｈｅ　surface coating of zeonex, looking for an effective way to verify the PEG layer <br>
* tried contact angle, device broken<br>
* SEM did not give out a lot of information<br>
* scheduling XPS<br>
* DNA chain model is being made (5 beads model tested).  <br>
† RCA<br>
* Cathie submitted One pager Whitepaper. Waiting for response. <br>
† HDA<br>
* Large volume filling and valve tested. <br>
* Design issue - whole integration or assemble parts (SPE & HDA chamber) <br>
<br>
† Senior project <br>
* Megan 1)did some preliminary bonding with Zeonex and teflon film.  2) emailed off the Mask Design to FineLine <br>
* <br>
† F31: Cochlea <br>
* Pre-gent vs. Post-gent-- most interesting data: decorin upregulation, fibronectin gradient <br>
* Six antibodies.  Verifying 4 with protein pre- and co-incubation with antibody.  Could not find the other two proteins <br>
* Finalized poster and printing for ARO
* ARO is next week
<br>
† Silica Optimization (Lambda): <br>
* Overview to look at the data collectively [http://openwetware.org/wiki/Image:Overview.xls] <br>
* Next E. coli. <br>
* try another color DNA dye. Pico green works ok
http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html
Specifically buy
http://products.invitrogen.com:80/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&productID=N7565&messageType=catProductDetail
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}


__NOTOC__
__NOTOC__

Revision as of 13:59, 12 February 2009

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

12 February 2009 Lab Meeting Agenda

  • Status: compressed air and vacuum? Why did the work not happen?

† Announcements

  • Sonali out for a week.
  • New senior project team.
  • MicroTAS abstracts.


† Flu R01

  • Modified Schedule this week.
  • Plaque Assay.


† SEPSIS Project

  • Sonali done with draft.

  • .


†RNA project

  • More formal report to Jeff from Cathie.
  • Do cDNA prep. Spec, gels, send to Jeff B. Shipping?


† COBRA

  • Mark- Control circuit - done, need to be tested.
  • Integration test from here forward br>

- Direct measurement with engineered SERS substrate.

  • Test with engineered SERS substrates

- 500umx500umx50um dimension informed to Luca and Bjoern
- Need to schedule when they are ready.

  • Substrates (JZ)

- Try glass fixture.
-
-

  • Colloid experiment. inconclusive. Look at SEMs

- difficulty in locating the bacteria
- needs much higher particle/bacteria ratio



† Valve Array/Valve building (FJ)

  • Teddy on track.
  • Valve tested


† Fraunhofer: LOAC

  • Paper drafting!


† Biointerfaces group


  • Final experiments in process? Need one more experiment next week


† CIMIT- Colson Grant

  • IRB still in revision.


† PCR

  • flu cDNA　ｗａｓ　ｓｕｃｃｅｓｓｆｕｌｌｙ　ａｍｐｌｉｆｉｅｄ　ｏｎ　ｃｈｉｐ，ｏｎｅ　ｂａｎｄ　ｓｈｏｗ　ｕｐ　ｉｎ　ｇｅｌ．　ｅｘｐｅｒｉｍｅｎｔ　ｈａｓ　ｂｅｅｎ　ｒｅｐｅａｔｅｄ　ｆｏｒ　ｔｈｒｅｅ　ｔｉｍｅ．
  • ｗｏｒｋｉｎｇ　ｏｎ　ｔｈｅ　surface coating of zeonex, looking for an effective way to verify the PEG layer
  • tried contact angle, device broken
  • SEM did not give out a lot of information
  • scheduling XPS
  • DNA chain model is being made (5 beads model tested).

† RCA

  • Cathie submitted One pager Whitepaper. Waiting for response.

† HDA

  • Large volume filling and valve tested.
  • Design issue - whole integration or assemble parts (SPE & HDA chamber)


† Senior project

  • Megan 1)did some preliminary bonding with Zeonex and teflon film. 2) emailed off the Mask Design to FineLine

† F31: Cochlea

  • Pre-gent vs. Post-gent-- most interesting data: decorin upregulation, fibronectin gradient
  • Six antibodies. Verifying 4 with protein pre- and co-incubation with antibody. Could not find the other two proteins
  • Finalized poster and printing for ARO
  • ARO is next week


† Silica Optimization (Lambda):

  • Overview to look at the data collectively [1]
  • Next E. coli.
  • try another color DNA dye. Pico green works ok

http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html

Specifically buy http://products.invitrogen.com:80/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&productID=N7565&messageType=catProductDetail


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Klapperich_Lab:Notebook/Lab_Meeting_Notes/2009/02/12&oldid=285569"