Klapperich Lab:Notebook/Lab Meeting Notes/2009/02/12

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(12 February 2009 Lab Meeting Agenda)
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† Biointerfaces group <br>
† Biointerfaces group <br>
* <br>
* <br>
-
* Final experiments in process? <br>
+
* Final experiments in process? Need one more experiment next week <br>
<br>
<br>
† CIMIT- Colson Grant<br>
† CIMIT- Colson Grant<br>
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* SEM did not give out a lot of information<br>
* SEM did not give out a lot of information<br>
* scheduling XPS<br>
* scheduling XPS<br>
 +
* DNA chain model is being made (5 beads model tested).  <br>
† RCA <br>
† RCA <br>
* Cathie submitted One pager Whitepaper. Waiting for response. <br>
* Cathie submitted One pager Whitepaper. Waiting for response. <br>

Revision as of 11:11, 12 February 2009

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12 February 2009 Lab Meeting Agenda

  • Status: compressed air and vacuum?

† Announcements

  • Sonali out for a week.
  • New senior project team.
  • MicroTAS abstracts.


† Flu R01

  • Modified Schedule this week.
  • Plaque Assay.
  • Alex needs to be trained to make SU molds. Trained to make the monoliths (by Hussam By 1/15/09)--we're about to start the training--.


† SEPSIS Project

  • Sonali done with draft.
  • Hussam working on Lambda phage data.
    .
  • 1 ml methanol wash seems to be taking care of the issue.


†RNA project

  • More formal report to Jeff from Cathie.
  • Do cDNA prep. Spec, gels, send to Jeff B. Shipping?

† COBRA

  • Mark- Control circuit - This will be done by January.
  • Evap quantification experiments ongoing. Still working on live/dead assay.(JD, JZ)

- problems: dead but green in the end
- called tech support.
- tests: supernatant fluorescent? excessive dye to observe time-lapse change in green/red ratio.
- omit washing step before dilution: wash, dye, incubate, dilute, instead of wash, dye, incubate, wash, dilute
- try running the experiment first, then running the live/dead dye through second. Bold text
- Petridish culture after running evaporation w/o dye in hydrophobic treated channel.

  • Hydrophobic coating of microchannel

- RIE (CHF3), Rain?, and Cytop coated and thermal bonding with PTFE filter tested.
- Cytop turned out good bonding with PTFE filter.

  • Test cheap wait for running
  • Slide for dispensing or integration scheme with SERS substrate


  • Substrates (JZ)

- new set made, in glove box: the 1st 3 on the DOE. SEM and SERS available.
- hypothesis: drying controls clustering, reduction controls size?

  • DOE for the experiment.

- need to add humidity as it changes even in the glove box - this is no longer an issue, asked Ranjith and now can adjust humidity to constant
- set II done varying drying time and fast reduction time. SEM and SERS available.

  • Colloid experiment. inconclusive. Look at SEMs

- difficulty in locating the bacteria
- needs much higher particle/bacteria ratio
- experiments outside the channel first?

  • Look at the unused substrates in SEM one week old or more.


† Valve Array/Valve building (FJ)

  • Teddy on track.


† Fraunhofer: LOAC

  • Paper drafting!


† Biointerfaces group


  • Final experiments in process? Need one more experiment next week


† CIMIT- Colson Grant

  • IRB still in revision.


† PCR

  • flu cDNA was successfully amplified on chip,one band show up in gel. experiment has been repeated for three time.
  • working on the surface coating of zeonex, looking for an effective way to verify the PEG layer
  • tried contact angle, device broken
  • SEM did not give out a lot of information
  • scheduling XPS
  • DNA chain model is being made (5 beads model tested).

† RCA

  • Cathie submitted One pager Whitepaper. Waiting for response.


† Senior project

  • Megan 1)did some preliminary bonding with Zeonex and teflon film. 2) emailed off the Mask Design to FineLine

† F31: Cochlea

  • Set up adviser meeting Jan
  • Post Gent vs. Pre gent - 6 proteins. 4/6 no change.
  • Final two proteins: know by week of 1/19


† Silica Optimization (Lambda):

  • Elution of C and D total DNA mass 2ng and 0.2 ng [1]
  • Testing with water 12 channels- standard protocol- NA replaced with NF water [2]
  • 10 channels - 5ch methanol-water / 5ch methanol-buffer-water [3]
  • Next E. coli.



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