Klapperich Lab:Notebook/Lab Meeting Notes/2009/02/12

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† Silica Optimization (Lambda): <br>
† Silica Optimization (Lambda): <br>
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* Elution of C and D total DNA mass 2ng and 0.2 ng  [http://openwetware.org/wiki/Image:C_and_D_results.pdf] <br>
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* Overview to look at the data collectively [http://openwetware.org/wiki/Image:Overview.xls] <br>
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* Testing with water 12 channels- standard protocol- NA replaced with NF water  [http://openwetware.org/wiki/Image:212808-12_samples_water_control_in_channels.pdf] <br>
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* 10 channels - 5ch methanol-water / 5ch methanol-buffer-water  [http://openwetware.org/wiki/Image:01-08-09_testing_methanol-buffer-water_in_channels.pdf] <br>
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* Next E. coli. <br>
* Next E. coli. <br>
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* try another color DNA dye.
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* try another color DNA dye. Pico green works ok
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http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html
http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html

Revision as of 16:00, 12 February 2009

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12 February 2009 Lab Meeting Agenda

  • Status: compressed air and vacuum?

† Announcements

  • Sonali out for a week.
  • New senior project team.
  • MicroTAS abstracts.


† Flu R01

  • Modified Schedule this week.
  • Plaque Assay.
  • Alex needs to be trained to make SU molds. Trained to make the monoliths (by Hussam By 1/15/09)--we're about to start the training--.


† SEPSIS Project

  • Sonali done with draft.
  • Hussam working on Lambda phage data.
    .
  • 1 ml methanol wash seems to be taking care of the issue.


†RNA project

  • More formal report to Jeff from Cathie.
  • Do cDNA prep. Spec, gels, send to Jeff B. Shipping?

† COBRA

  • Mark- Control circuit - done, need to be tested.
  • Evap quantification experiments.(JD, JZ)

- Petridish culture after running evaporation w/o dye in hydrophobic treated channel
- ; Viability low as < 20%, Huge variation in results
- try running the experiment first, then running the live/dead dye through second. Bold text
- ; Live/dead assay kills 90% bacteria proven by test.
- Direct measurement with engineered SERS substrate.

  • Test with engineered SERS substrates

- 500umx500umx50um dimension informed to Luca and Bjoern
- Need to schedule when they are ready

  • Substrates (JZ)

- new set made, in glove box: the 1st 3 on the DOE. SEM and SERS available.
- hypothesis: drying controls clustering, reduction controls size?

  • DOE for the experiment.

- need to add humidity as it changes even in the glove box - this is no longer an issue, asked Ranjith and now can adjust humidity to constant
- set II done varying drying time and fast reduction time. SEM and SERS available.

  • Colloid experiment. inconclusive. Look at SEMs

- difficulty in locating the bacteria
- needs much higher particle/bacteria ratio
- experiments outside the channel first?

  • Look at the unused substrates in SEM one week old or more.


† Valve Array/Valve building (FJ)

  • Teddy on track.
  • Valve tested


† Fraunhofer: LOAC

  • Paper drafting!


† Biointerfaces group


  • Final experiments in process? Need one more experiment next week


† CIMIT- Colson Grant

  • IRB still in revision.


† PCR

  • flu cDNA was successfully amplified on chip,one band show up in gel. experiment has been repeated for three time.
  • working on the surface coating of zeonex, looking for an effective way to verify the PEG layer
  • tried contact angle, device broken
  • SEM did not give out a lot of information
  • scheduling XPS
  • DNA chain model is being made (5 beads model tested).

† RCA

  • Cathie submitted One pager Whitepaper. Waiting for response.

† HDA

  • Large volume filling and valve tested.
  • Design issue - whole integration or assemble parts (SPE & HDA chamber)


† Senior project

  • Megan 1)did some preliminary bonding with Zeonex and teflon film. 2) emailed off the Mask Design to FineLine

† F31: Cochlea

  • Pre-gent vs. Post-gent-- most interesting data: decorin upregulation, fibronectin gradient
  • Six antibodies. Verifying 4 with protein pre- and co-incubation with antibody. Could not find the other two proteins
  • Finalized poster and printing for ARO
  • ARO is next week


† Silica Optimization (Lambda):

  • Overview to look at the data collectively [1]
  • Next E. coli.
  • try another color DNA dye. Pico green works ok

http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html

Specifically buy http://products.invitrogen.com:80/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&productID=N7565&messageType=catProductDetail


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