12 February 2009 Lab Meeting Agenda
- Status: compressed air and vacuum?
† Announcements
- Sonali out for a week.
- New senior project team.
- MicroTAS abstracts.
† Flu R01
- Modified Schedule this week.
- Plaque Assay.
- Alex needs to be trained to make SU molds. Trained to make the monoliths (by Hussam By 1/15/09)--we're about to start the training--.
† SEPSIS Project
- Sonali done with draft.
- Hussam working on Lambda phage data.
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- 1 ml methanol wash seems to be taking care of the issue.
†RNA project
- More formal report to Jeff from Cathie.
- Do cDNA prep. Spec, gels, send to Jeff B. Shipping?
† COBRA
- Mark- Control circuit - This will be done by January.
- Evap quantification experiments ongoing. Still working on live/dead assay.(JD, JZ)
- problems: dead but green in the end
- called tech support.
- tests: supernatant fluorescent? excessive dye to observe time-lapse change in green/red ratio.
- omit washing step before dilution: wash, dye, incubate, dilute, instead of wash, dye, incubate, wash, dilute
- try running the experiment first, then running the live/dead dye through second. Bold text
- Petridish culture after running evaporation w/o dye in hydrophobic treated channel.
- Hydrophobic coating of microchannel
- RIE (CHF3), Rain?, and Cytop coated and thermal bonding with PTFE filter tested.
- Cytop turned out good bonding with PTFE filter.
- Test cheap wait for running
- Slide for dispensing or integration scheme with SERS substrate
- new set made, in glove box: the 1st 3 on the DOE. SEM and SERS available.
- hypothesis: drying controls clustering, reduction controls size?
- need to add humidity as it changes even in the glove box - this is no longer an issue, asked Ranjith and now can adjust humidity to constant
- set II done varying drying time and fast reduction time. SEM and SERS available.
- Colloid experiment. inconclusive. Look at SEMs
- difficulty in locating the bacteria
- needs much higher particle/bacteria ratio
- experiments outside the channel first?
- Look at the unused substrates in SEM one week old or more.
† Valve Array/Valve building (FJ)
† Fraunhofer: LOAC
† Biointerfaces group
- Final experiments in process? Need one more experiment next week
† CIMIT- Colson Grant
† PCR
- flu cDNA ｗａｓ ｓｕｃｃｅｓｓｆｕｌｌｙ ａｍｐｌｉｆｉｅｄ ｏｎ ｃｈｉｐ，ｏｎｅ ｂａｎｄ ｓｈｏｗ ｕｐ ｉｎ ｇｅｌ． ｅｘｐｅｒｉｍｅｎｔ ｈａｓ ｂｅｅｎ ｒｅｐｅａｔｅｄ ｆｏｒ ｔｈｒｅｅ ｔｉｍｅ．
- ｗｏｒｋｉｎｇ ｏｎ ｔｈｅ surface coating of zeonex, looking for an effective way to verify the PEG layer
- tried contact angle, device broken
- SEM did not give out a lot of information
- scheduling XPS
- DNA chain model is being made (5 beads model tested).
† RCA
- Cathie submitted One pager Whitepaper. Waiting for response.
† Senior project
- Megan 1)did some preliminary bonding with Zeonex and teflon film. 2) emailed off the Mask Design to FineLine
† F31: Cochlea
- Pre-gent vs. Post-gent-- most interesting data: decorin upregulation, fibronectin gradient
- Six antibodies. Verifying 4 with protein pre- and co-incubation with antibody. Could not find the other two proteins
- Finalized poster and printing for ARO
- ARO is next week
† Silica Optimization (Lambda):
- Elution of C and D total DNA mass 2ng and 0.2 ng [1]
- Testing with water 12 channels- standard protocol- NA replaced with NF water [2]
- 10 channels - 5ch methanol-water / 5ch methanol-buffer-water [3]
- Next E. coli.
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