12 February 2009 Lab Meeting Agenda
- Status: compressed air and vacuum?
† Announcements
- Sonali out for a week.
- New senior project team.
- MicroTAS abstracts.
† Flu R01
- Modified Schedule this week.
- Plaque Assay.
- Alex needs to be trained to make SU molds. Trained to make the monoliths (by Hussam By 1/15/09)--we're about to start the training--.
† SEPSIS Project
- Sonali done with draft.
- Hussam working on Lambda phage data.
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- 1 ml methanol wash seems to be taking care of the issue.
†RNA project
- More formal report to Jeff from Cathie.
- Do cDNA prep. Spec, gels, send to Jeff B. Shipping?
† COBRA
- Mark- Control circuit - done, need to be tested.
- Evap quantification experiments.(JD, JZ)
- Petridish culture after running evaporation w/o dye in hydrophobic treated channel
- ; Viability low as < 20%, Huge variation in results
- try running the experiment first, then running the live/dead dye through second. Bold text
- ; Live/dead assay kills 90% bacteria proven by test.
- Direct measurement with engineered SERS substrate.
- Test with engineered SERS substrates
- 500umx500umx50um dimension informed to Luca and Bjoern
- Need to schedule when they are ready
- new set made, in glove box: the 1st 3 on the DOE. SEM and SERS available.
- hypothesis: drying controls clustering, reduction controls size?
- need to add humidity as it changes even in the glove box - this is no longer an issue, asked Ranjith and now can adjust humidity to constant
- set II done varying drying time and fast reduction time. SEM and SERS available.
- Colloid experiment. inconclusive. Look at SEMs
- difficulty in locating the bacteria
- needs much higher particle/bacteria ratio
- experiments outside the channel first?
- Look at the unused substrates in SEM one week old or more.
† Valve Array/Valve building (FJ)
- Teddy on track.
- Valve tested
† Fraunhofer: LOAC
† Biointerfaces group
- Final experiments in process? Need one more experiment next week
† CIMIT- Colson Grant
† PCR
- flu cDNA ｗａｓ ｓｕｃｃｅｓｓｆｕｌｌｙ ａｍｐｌｉｆｉｅｄ ｏｎ ｃｈｉｐ，ｏｎｅ ｂａｎｄ ｓｈｏｗ ｕｐ ｉｎ ｇｅｌ． ｅｘｐｅｒｉｍｅｎｔ ｈａｓ ｂｅｅｎ ｒｅｐｅａｔｅｄ ｆｏｒ ｔｈｒｅｅ ｔｉｍｅ．
- ｗｏｒｋｉｎｇ ｏｎ ｔｈｅ surface coating of zeonex, looking for an effective way to verify the PEG layer
- tried contact angle, device broken
- SEM did not give out a lot of information
- scheduling XPS
- DNA chain model is being made (5 beads model tested).
† RCA
- Cathie submitted One pager Whitepaper. Waiting for response.
† HDA
- Large volume filling and valve tested.
- Design issue - whole integration or assemble parts (SPE & HDA chamber)
† Senior project
- Megan 1)did some preliminary bonding with Zeonex and teflon film. 2) emailed off the Mask Design to FineLine
† F31: Cochlea
- Pre-gent vs. Post-gent-- most interesting data: decorin upregulation, fibronectin gradient
- Six antibodies. Verifying 4 with protein pre- and co-incubation with antibody. Could not find the other two proteins
- Finalized poster and printing for ARO
- ARO is next week
† Silica Optimization (Lambda):
- Elution of C and D total DNA mass 2ng and 0.2 ng [1]
- Testing with water 12 channels- standard protocol- NA replaced with NF water [2]
- 10 channels - 5ch methanol-water / 5ch methanol-buffer-water [3]
- Next E. coli.
- try another color DNA dye.
http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html
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