Klapperich Lab:Notebook/Lab Meeting Notes/2009/02/19: Difference between revisions

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†RNA project
†RNA project
* Samples Sent? <br>
* Samples Sent? <br>
*  <br>
*  New proposal with Fraunhofer for food. <br>
<br>
 
<br>
<br>
† COBRA <br>
† COBRA <br>
* Mark- Control circuit - done, need to be tested.<br>  
* Mark- Control circuit - done, need to be tested.<br>  
* Integration test from here forward <br>
* Integration test from here forward. <br>
- Direct measurement with engineered SERS substrate.<br>  
- Direct measurement with engineered SERS substrate.<br>  
* Test with engineered SERS substrates <br>
* Test with engineered SERS substrates <br>
-  Luca and Bjoern <br>
* Flu Concentration and Cleaning. Kit, UCentrifuge, Quote from Beckmann. <br>
<br>
<br>
* Substrates (JZ)<br>
* Substrates (JZ)<br>
- Try glass fixture. <br>
- Try glass fixture. <br>
- <br>
- <br>
- <br>
- <br>
* Colloid experiment. inconclusive. Look at SEMs <br>
* Colloid experiment. <br>
- difficulty in locating the bacteria <br>
- Do Rhodamine. <br>
- needs much higher particle/bacteria ratio <br>
-Need a size specific positive control. PS beads? charged? <br>
*<br>
*<br>
<br>
<br>
† Valve Array/Valve building (FJ) <br>
† Valve Array/Valve building. <br>
*  <br>
*  Parts missing. Check with Susan.<br>
* Valve tested <br>
* Writing a new control interface. <br>
<br>
<br>
† Fraunhofer: LOAC <br>
† Fraunhofer: LOAC <br>
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† Biointerfaces group <br>
† Biointerfaces group <br>
* <br>
* <br>
* Final experiments in process? Need one more experiment next week <br>
* Final experiments were done. <br>
* counting the number of trapped cells <br>
* adding experimental results in the draft paper.<br>
<br>
<br>
† CIMIT- Colson Grant<br>
† CIMIT- Colson Grant<br>
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† PCR <br>
† PCR <br>
* surface coating of zeonex, looking for an effective way to verify the PEG layer <br>
* surface coating of zeonex, looking for an effective way to verify the PEG layer <br>
* tried contact angle, device broken<br>
* tried contact angle, device is still broken<br>
* SEM did not give out a lot of information<br>
* SEM showed no pattern on the plain zeonex without plasma treatment<br>
* XPS? <br>
* XPS? <br>
* DNA chain model is being made (5 beads model tested).  <br>
* detect roughness with zygo<br>
* Simulaons of DNA chain model are running (60 beads).  <br>
<br>
<br>
† RCA<br>
† RCA<br>
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† Senior project <br>
† Senior project <br>
* Megan<br>  
* Megan<br>  
* <br>
* Cutter plotter for fast prototype.<br>
* Need to get back in cleanroom. Hussam?  Get 3" wafers from MCK. <br>
<br>
† F31: Cochlea <br>
† F31: Cochlea <br>
<br>
* Nathan out this week.<br>
* Nathan out this week.<br>
<br>
<br>
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* try another color DNA dye.
* try another color DNA dye.
*<b>UPDATE The invitrogen Nucleic Acid Stains Dimer Sampler Kit was ordered on Tuesday </b>


http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html
http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html
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Specifically buy
Specifically buy
http://products.invitrogen.com:80/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&productID=N7565&messageType=catProductDetail
http://products.invitrogen.com:80/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&productID=N7565&messageType=catProductDetail
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<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
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__NOTOC__
__NOTOC__

Revision as of 14:00, 19 February 2009

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19 February 2009 Lab Meeting Agenda

  • Status: compressed air and vacuum. Happening NOW.

† Announcements

  • MicroTAS abstracts.


† Flu R01

  • Modified Schedule this week.
  • Plaque Assay.


† SEPSIS Project

  • Submitted to Integrative Biology.

  • .


†RNA project

  • Samples Sent?
  • New proposal with Fraunhofer for food.


† COBRA

  • Mark- Control circuit - done, need to be tested.
  • Integration test from here forward.

- Direct measurement with engineered SERS substrate.

  • Test with engineered SERS substrates
  • Flu Concentration and Cleaning. Kit, UCentrifuge, Quote from Beckmann.


  • Substrates (JZ)

- Try glass fixture.
-
-

  • Colloid experiment.

- Do Rhodamine.
-Need a size specific positive control. PS beads? charged?



† Valve Array/Valve building.

  • Parts missing. Check with Susan.
  • Writing a new control interface.


† Fraunhofer: LOAC

  • Paper drafting!


† Biointerfaces group


  • Final experiments were done.
  • counting the number of trapped cells
  • adding experimental results in the draft paper.


† CIMIT- Colson Grant

  • IRB still in revision.


† PCR

  • surface coating of zeonex, looking for an effective way to verify the PEG layer
  • tried contact angle, device is still broken
  • SEM showed no pattern on the plain zeonex without plasma treatment
  • XPS?
  • detect roughness with zygo
  • Simulaons of DNA chain model are running (60 beads).


† RCA

  • Cathie submitted One pager Whitepaper. Waiting for response.


† HDA

  • Large volume filling and valve tested.
  • Design issue - whole integration or assemble parts (SPE & HDA chamber)


† Senior project

  • Megan
  • Cutter plotter for fast prototype.
  • Need to get back in cleanroom. Hussam? Get 3" wafers from MCK.


† F31: Cochlea

  • Nathan out this week.


† Silica Optimization (Lambda):

  • Overview to look at the data collectively [1]
  • Next E. coli.
  • try another color DNA dye.
  • UPDATE The invitrogen Nucleic Acid Stains Dimer Sampler Kit was ordered on Tuesday

http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html

Specifically buy http://products.invitrogen.com:80/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&productID=N7565&messageType=catProductDetail



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